A RAPID AND EFFICIENT PROCEDURE FOR THE PURIFICATION OF DNA FROM AGAROSE GELS
A RAPID AND EFFICIENT PROCEDURE FOR THE PURIFICATION OF DNA FROM AGAROSE GELS
复制标题
DOI：
10.1016/0003-2697(80)90553-9
复制
发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
GRAHAM, FL
中科院分区：
文献类型：
--
作者：
GIRVITZ, SC;BACCHETTI, S;GRAHAM, FL
通过水平琼脂糖平板凝胶电泳的DNA片段可以通过将透析膜支持的滤纸条插入DNA条带前面的凝胶中切割的狭缝中并继续电泳直到DNA收集在纸上来回收。通过低速离心从滤纸上洗脱DNA。用该技术常规地获得远高于70%的回收率，并且回收的DNA具有生物活性，并且可以通过切口平移在体外进行再切割、连接、标记并与RNA杂交。
DNA fragments electrophoresed through a horizontal agarose slab gel can be recovered by inserting strips of filter paper backed by dialysis membrane into slits cut in the gel in front of the DNA bands and continuing electrophoresis until the DNA is collected in the paper. Elution of the DNA from the filter paper is achieved by low-speed centrifugation. Recovery well above 70% is routinely obtained with this technique and the DNA recovered is biologically active and can be recleaved, ligated, labeled in vitro by nick translation and hybridized to RNA.