Detoxification of malachite green and textile industrial effluent by Penicillium ochrochloron
Detoxification of malachite green and textile industrial effluent by Penicillium ochrochloron
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DOI：
10.1007/s12257-010-0069-0
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发表时间：
2011-04
影响因子：
3.2
通讯作者：
Utkarsha U. Shedbalkar;J. Jadhav
中科院分区：
文献类型：
--
作者：
Utkarsha U. Shedbalkar;J. Jadhav
孔雀石绿色经绿黄青霉菌（Penicillium ochrochloron）脱毒后，生成对苄基-N，N-二甲基苯胺和N，N-二甲基苯胺盐酸盐。通过TLC、HPLC和FTIR分析降解代谢产物，并通过GCMS分析进行鉴别。植物毒性试验表明，这些代谢产物的无毒性质。在30°C、pH 7条件下，对50 mg/L孔雀石绿色的脱色率为93%。降解后木质素过氧化物酶活性的诱导表明孔雀石绿色的降解是过氧化物酶介导的。真菌培养也被发现对纺织废水有解毒作用。TDS，TSS，COD和BOD的值降低处理后的样品相比，控制出水。处理后的污水对小麦和Ervum lensLinn的植株无毒害作用，处理后的污水中叶绿素总量高于对照污水。
Malachite green was detoxified into p-benzyl-N,N-dimethylaniline and N,N-dimethyl-aniline hydrochloride byPenicillium ochrochloron. Degradation metabolites were analyzed by TLC, HPLC, and FTIR and identified by GCMS analysis. Phytotoxicity testing revealed the nontoxic nature of these metabolites. The percentage decolorization of malachite green (50 mg/L) was 93% in czapek dox broth after 14 h with an optimum pH of 7 at 30°C. The induction in the activity of lignin peroxidase after degradation suggested that the degradation of malachite green was peroxidase-mediated. Fungal culture was also found to have detoxified the textile effluent. The values of TDS, TSS, COD, and BOD were reduced in the treated samples compared to the control effluent. The treated effluent was non-toxic to the plants ofTriticum aestivumandErvum lensLinn, and the amount of total chlorophyll was higher in plants with treated effluent when compared to control effluent.