Attachment of plasma membrane vesicles of human macrophages to Leishmania tropica promastigotes.
Attachment of plasma membrane vesicles of human macrophages to Leishmania tropica promastigotes.
复制标题
人巨噬细胞质膜囊泡与热带利什曼原虫前鞭毛体的附着。
DOI：
10.1093/infdis/148.3.377
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Wyler,DJ
中科院分区：
文献类型：
--
作者：
Klempner,MS;Cendron,M;Wyler,DJ
Intracellular parasitism of host macrophages is the pathologic hallmark of leishmaniasis. Since the organisms are found almost exclusivelyin this type of cell, the possibility that specific macrophage plasma-membrane determinants mediate the attachment to promastigotes ofLeishmania tropicawas investigated. Plasma membrane vesicleswere prepared from human monocytes/macrophages and erythrocytes, radioiodinated, and incubated withL tropicapromastigotes, erythrocytes, or Sephadex beads. Macrophage plasma-membrane vesicles bound to promastigotes to a significant extent but did not bind to intact erythrocytes or to an inert particle. In contrast, erythrocyte membrane vesicles did not bind to promastigotes. The binding of macrophage plasma-membrane vesicles to promastigotes demonstrated the characteristics of a receptor-ligand interaction in terms of specificity, saturability, competitive inhibition, and temperature independence. These results suggest the presence of one or more intrinsic binding sites on the macrophage plasma membrane to which promastigotes can attach. If this is the case, therapeutic intervention by strategies that inhibit attachment of this obligate intracellular parasite to its target host cell may be possible.
