Oxidized galectin-1 stimulates the migration of Schwann cells from both proximal and distal stumps of transected nerves and promotes axonal regeneration after peripheral nerve injury
Oxidized galectin-1 stimulates the migration of Schwann cells from both proximal and distal stumps of transected nerves and promotes axonal regeneration after peripheral nerve injury
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DOI：
10.1093/jnen/62.2.162
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发表时间：
2003-02-01
影响因子：
3.2
通讯作者：
Yamashita, J
中科院分区：
文献类型：
--
作者：
Fukaya, K;Hasegawa, M;Yamashita, J
氧化半乳糖凝集素-1最近已被确定为在损伤的周围神经的初始轴突生长中起重要作用的关键因子。本研究的目的是研究氧化半乳糖凝集素-1对大鼠脊神经再生的影响，使用脱细胞自体移植物（不含活细胞）和同种异体移植物（不含细胞膜），特别注意轴突再生和雪旺细胞迁移之间的关系。免疫组化结果显示，内源性半乳糖凝集素1在正常坐骨神经的背根神经节（DRG）神经元、脊髓运动神经元、轴突和雪旺细胞中均有表达。在自体移植模型中给予氧化重组人半乳糖凝集素-1（rh-gal-1 ox，5 ng/ml）促进了运动神经元以及DRG神经元的轴突再生;这一点通过荧光金示踪剂研究证实（p < 0.05）。抗rh-gal-1抗体（30 μ g/ml）强烈抑制轴突.再生长（p < 0.05）。用rh-gal-1 ox预处理移植物可促进雪旺细胞从近端和远端向移植物内迁移，从而加速轴突再生（p < 0.05）。此外，雪旺细胞迁移之前的轴突生长在移植物中存在外源性rh-gal-1 ox。这些结果有力地表明，局部施用外源性rh-gal-1 ox促进雪旺细胞的迁移，随后促进运动神经元和感觉神经元的轴突再生，从而加速神经元修复。这项技术在修复人类神经方面也有价值。
Oxidized galectin-1 has recently been identified as a key factor that plays important roles in initial axonal growth in injured peripheral nerves. The aim of this study was to investigate the effects of oxidized galectin-1 on regeneration of rat spinal nerves using acellular autografts (containing no viable cells) and allografts (containing no cell membranes) with special attention to the relationship between axonal regeneration and Schwann cell migration. Immunohistochemically, endogenous galectin-1 was expressed in dorsal root ganglion (DRG) neurons, spinal cord motoneurons, and axons and Schwann cells in normal sciatic nerves. Administration of oxidized recombinant human galectin-1 (rh-gal-1ox, 5 ng/ml) in autograft model promoted axonal regeneration from motoneurons as well as from DRG neurons; this was confirmed by a fluorogold tracer study (p < 0.05). Anti-rh-gal-1 antibody (30 mug/ml) strongly inhibited axonal. regrowth (p < 0.05). Pretreatment of allografts with rh-gal-1ox stimulated the migration of Schwann cells not only from proximal stumps but also from distal stumps into the grafts, resulting in accelerated axonal regeneration (p < 0.05). Moreover, Schwann cell migration preceded the axonal growth in the presence of exogenous rh-gal-1ox in the grafts. These results strongly suggest that local administration of exogenous rh-gal-1ox promotes the migration of Schwann cells followed by axonal regeneration from both motor and sensory neurons, resulting in acceleration of neuronal repair. This technique may also be of value in the repair of human nerves.