Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis
Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis
复制标题
删除 HAPS_2096 会增加副猪嗜血杆菌对天蚕素 B 的敏感性
DOI：
10.1159/000434752
复制
发表时间：
2015-01-01
影响因子：
1.2
通讯作者：
Cao, Jiyue
中科院分区：
文献类型：
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作者：
Chen, Fanjie;Hu, Han;Cao, Jiyue
天蚕素 B (CB) 是一种非常有效的天然抗菌肽，在未来抗菌药物的开发中显示出巨大的潜力。 HAPS_2096 是副猪嗜血杆菌基因，编码 ATP 结合盒型氨基酸转运蛋白的周质底物结合蛋白。在本研究中，我们构建并验证了副猪嗜血菌JS0135的HAPS_2096缺失突变体和互补的HAPS_2096突变体。杀菌试验表明，HAPS_2096 缺失突变体对 0.25-0.5 µg/ml CB 的敏感性显着高于野生型菌株。然而，基因互补减轻了突变体的CB敏感性。用亚致死浓度的 CB (0.25 μg/ml) 处理 30 分钟后的免疫电镜观察显示，突变菌株比野生型菌株出现更广泛的形态损伤。因此，我们的结果表明 HAPS_2096 基因有助于副猪嗜血菌对 CB 的抗性。
Cecropin B (CB) is a very effective natural antimicrobial peptide that has shown great potential for future antimicrobial drug development. HAPS_2096 is a Haemophilus parasuis gene that encodes the periplasmic substrate-binding protein of an ATP-binding cassette-type amino acid transporter. In this research, we constructed and verified an HAPS_2096 deletion mutant and a complementary HAPS_2096 mutant of H. parasuis JS0135. A bactericidal assay revealed that the HAPS_2096 deletion mutant was significantly more sensitive than the wild-type strain to 0.25-0.5 µg/ml CB. However, the gene complementation alleviated the CB sensitivity of the mutant. Immunoelectron microscopy observation following a 30-min treatment with a sublethal concentration of CB (0.25 μg/ml) revealed more extensive morphological damage in the mutant strain than in the wild-type strain. Hence, our results suggest that the HAPS_2096 gene contributes to H. parasuis resistance to CB.