Aptamer biosensor for protein detection using gold nanoparticles
Aptamer biosensor for protein detection using gold nanoparticles
复制标题
使用金纳米颗粒进行蛋白质检测的适体生物传感器
DOI：
10.1016/j.ab.2007.11.013
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发表时间：
2008-02-15
影响因子：
2.9
通讯作者：
Zhao, Xin Sheng
中科院分区：
文献类型：
--
作者：
Wang, Wenjuan;Chen, Chunlai;Zhao, Xin Sheng
以金纳米粒子为荧光猝灭剂，核酸适体为探针，利用DNA修饰的金纳米粒子构建了蛋白质生物传感器。我们研究了实验设计，如DNA链和GNP之间的相互作用的类型，温度和适体的微环境，如何影响生物传感器的识别能力。在我们的实验条件下，染料标记的DNA与固定在GNP上的适体杂交的复合物（Ap-Im-GNP）对蛋白质的识别显示出最好的蛋白质检测特性。(C)2007爱思唯尔公司All rights reserved.
Combining gold nanoparticles (GNPs) as fluorescence quencher and aptamer as probe, we have developed protein biosensors by using DNA-modified GNPs. We examined how the experimental design, such as the type of interaction between DNA strands and GNPs, temperature, and microenvironment of aptamer, influences the recognition ability of the biosensor. Under our experimental conditions, the recognition of protein by the complex of dye-labeled DNA hybridized with aptamer that is immobilized on GNPs (Ap-Im-GNPs) shows the best character in protein detection. (C) 2007 Elsevier Inc. All rights reserved.