Ruthenium red staining of vaginal epithelial cells and adherent bacteria.
Ruthenium red staining of vaginal epithelial cells and adherent bacteria.
复制标题
阴道上皮细胞和粘附细菌的钌红染色。
DOI：
10.1002/ar.1092120106
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
King,BF
中科院分区：
文献类型：
--
作者：
King,BF
恒河猴的阴道上皮在整个月经周期和怀孕早期都是角化的复层鳞状上皮。表面细胞具有增厚的细胞包膜、表面微脊和大量粘附细菌。在怀孕后期，细胞粘液化并具有典型的细胞膜、微绒毛，并且没有粘附细菌。在本研究中，我们通过检查钌红染色后的阴道表面结构来扩展这些观察结果。在整个周期中，表面细胞有一层薄薄的染色物质与细胞膜紧密相连，但在某些情况下观察到更厚的粘液层。在妊娠后期，上皮细胞具有与细胞膜相关的中等厚度、稍微聚集的钌红阳性物质。许多细菌表面的糖萼成分也被染上钌红。许多类型的细菌对阴道上皮细胞的粘附似乎受到细菌和上皮细胞表面上的聚阴离子成分的相互作用的影响。
The vaginal epithelium of the rhesus monkey is a keratinized stratified squamous epithelium throughout the menstrual cycle and early pregnancy. The superficial cells have a thickened cell envelope, surface microridges, and numerous adherent bacteria. During later pregnancy the cells mucify and have a typical cell membrane, microvilli, and no adherent bacteria. In the present study we have extended these observations by examining vaginal surface structures after ruthenium red staining. Throughout the cycle, the superficial cells have a thin layer of stained material closely associated with the cell membrane, but in some cases a much thicker mucous blanket was observed. During later pregnancy the epithelial cells had a moderately thick, somewhat clumped ruthenium red‐positive material associated with the cell membrane. Glycocalyx components of the surface of many bacteria also stained with ruthenium red. The adherence of many types of bacteria to the vaginal epithelial cells appears to be effected by the interaction of polyanionic components on the surface of both the bacterial and epithelial cells.