Effects of temperature on the susceptibility of largemouth bass to largemouth bass virus
Effects of temperature on the susceptibility of largemouth bass to largemouth bass virus
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DOI：
10.1577/h03-009
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发表时间：
2003-09-01
影响因子：
1.2
通讯作者：
Goldberg, TL
中科院分区：
文献类型：
--
作者：
Grant, EC;Philipp, DP;Goldberg, TL
温度是一个环境变量，被认为会影响鱼类对传染病的易感性。这项研究表明，在实验中感染大口黑鲈病毒（LMBV;虹彩病毒科）的幼年大口黑鲈在30 ℃下的死亡率高于25 ℃。幼大口黑鲈暴露于相同剂量的LMBV，并保持在三个温度：25，30和35 ℃。在30摄氏度下饲养的鱼比在25摄氏度下饲养的鱼死亡率更高，死亡时的病毒载量（每克组织中的病毒基因组）也更高。LMBV注射的鱼在35 degreesC下的死亡率与假注射对照组的死亡率相等，这表明在接近致死上限的温度下的应激操作可以诱导鱼的死亡率，而与病毒感染无关。这些结果表明，温度是一个重要的决定因素，宿主的生存和病毒复制的LMBV系统。
Temperature is an environmental variable thought to influence the susceptibility of fish to infectious diseases. This study demonstrated that juvenile largemouth bass Micropterus salmoides that were experimentally infected with largemouth bass virus (LMBV; family Iridoviridae) experienced greater mortality at 30degreesC than at 25degreesC. Juvenile largemouth bass were exposed to equal doses of LMBV and held at three temperatures: 25, 30, and 35degreesC. Fish held at 30degreesC suffered mortality at a higher rate than fish held at 25degreesC and had higher viral loads (viral genomes per gram of tissue) at the time of death. The LMBV-injected fish held at 35degreesC suffered mortality at rates equal to those of sham-injected controls, suggesting that stressful manipulation at temperatures approaching the upper lethal limit can induce mortality in fish independently of viral infection. These results suggest that temperature is an important determinant of host survival and viral replication in the LMBV system.