Control of cell adhesion and detachment on Langmuir-Schaefer surface composed of dodecyl-terminated thermo-responsive polymers
Control of cell adhesion and detachment on Langmuir-Schaefer surface composed of dodecyl-terminated thermo-responsive polymers
复制标题
由十二烷基封端热响应聚合物组成的 Langmuir-Schaefer 表面细胞粘附和脱离的控制
DOI：
10.1080/09205063.2013.866761
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Okano T.
中科院分区：
文献类型：
--
作者：
Sakuma M;Kumashiro Y;Nakayama M;Tanaka N;Umemura K;Yamato M;Okano T.
采用Langmuir-Schaefer（LS）法制备了温敏性聚N-异丙基丙烯酰胺（PIPAAm）修饰表面。采用可逆加成-断裂链转移自由基聚合法合成了十二烷基封端的PIPAAm（PIPAAm-C_（12））。将PIPAAm-C12滴加到空气-水界面上并通过压缩形成朗缪尔膜。表面压力测量显示PIPAAm-C12漂浮并且在界面上形成Langmuir膜。并且将Langmuir膜转移到疏水基底上以产生PIPAAm-C12转移表面（PIPAAm-LS表面）。原子力显微镜、衰减全反射傅里叶变换红外光谱仪和X射线光电子能谱仪的测量结果表明，LB膜具有迁移性，密度可以精确控制。PIPAAm-LS表面的椭圆偏振测量表明表面的厚度小于10 nm。在PIPAAm-LS表面上观察到细胞粘附和脱离。发现在37 °C下，所有LS表面上的粘附细胞的量在对照疏水基底上相似。关于细胞脱离，粘附细胞以较高密度和较短的PIPAAm-C12分子自身快速脱离。在该方法中，观察了密度和分子量对细胞粘附和脱离的影响。我们的方法应该被证明是新的见解调查细胞粘附和脱离的温度响应表面。
This study used Langmuir-Schaefer (LS) method to produce thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) modified surface. Dodecyl terminated-PIPAAm (PIPAAm-C12) was synthesized by reversible addition-fragmentation chain transfer radical polymerization. PIPAAm-C12 was dropped on an air–water interface and formed Langmuir film by compressing. A surface pressure measurement revealed that PIPAAm-C12 was floated and Langmuir films were formed on the interface. And the Langmuir film was transferred on a hydrophobic substrate to produce PIPAAm-C12 transferred surface (PIPAAm-LS surface). In the results of atomic force microscope, attenuated total reflection Fourier transform infrared spectroscope, and X-ray photoelectron spectroscope measurement, the transference of Langmuir films was demonstrated and densities could be precisely controlled. Ellipsometric measurements of PIPAAm-LS surfaces showed that the thicknesses of the surfaces were less than 10 nm. Cell adhesion and detachment were observed on the PIPAAm-LS surfaces. The amount of adhered cells on all LS surfaces was found to be similar on the control hydrophobic substrate at 37 °C. In regard to cell detachment, adhering cells rapidly detached themselves with higher densities and shorter PIPAAm-C12 molecules. In this method, the effect of densities and molecular weights on cell adhesion and detachment were observed. Our method should be proved novel insights for investigating cell adhesion and detachment on thermo-responsive surfaces.