Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes
Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probes
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DOI：
10.1021/nl080358
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发表时间：
2008-05-01
期刊：
影响因子：
10.8
通讯作者：
Bao, Gang
中科院分区：
文献类型：
--
作者：
Dennis, Allison M.;Bao, Gang
荧光共振能量转移 (FRET) 特征，包括六个荧光蛋白 (FP)-量子点 (OD) 对的效率、供体-受体距离和结合强度进行了量化，表明 FP 是 QD 供体的有效受体，QD 荧光猝灭高达 90%，并且多组氨酸与 OD 核-壳表面的配位是将蛋白质与市售 QD 缀合的一种简单有效的方法。这为开发用于生物医学应用的基于 QD 的 FRET 探针提供了一种新方法。
Fluorescence resonance energy transfer (FRET) characteristics, including the efficiency, donor-acceptor distance, and binding strength of six fluorescent protein (FP)-quantum dot (OD) pairs were quantified, demonstrating that FPs are efficient acceptors for QD donors with up to 90% quenching of QD fluorescence and that polyhistidine coordination to OD core-shell surface is a straightforward and effective means of conjugating proteins to commercially available QDs. This provides a novel approach to developing QD-based FRET probes for biomedical applications.