POSTTRANSCRIPTIONAL MODIFICATION OF TRANSFER-RNA IN THERMOPHILIC ARCHAEA (ARCHAEBACTERIA)
POSTTRANSCRIPTIONAL MODIFICATION OF TRANSFER-RNA IN THERMOPHILIC ARCHAEA (ARCHAEBACTERIA)
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DOI：
10.1128/jb.173.10.3138-3148.1991
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发表时间：
1991-05-01
影响因子：
3.2
通讯作者：
MCCLOSKEY, JA
中科院分区：
文献类型：
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作者：
EDMONDS, CG;CRAIN, PF;MCCLOSKEY, JA
对11株嗜热古菌的未分级tRNA进行了核苷修饰研究（古细菌），包括嗜热产甲烷菌和硫代谢超嗜热菌的遗传多样性代表，其在56 ℃的温度范围内生长最佳（嗜酸热浆菌）至105 ℃（Pyrodictium occultum），并与已知的最嗜热细菌（真细菌）海栖热袍菌（Thermotoga maritima）（80 ℃）进行比较。 九个核苷被发现是独特的古菌，其中六个是结构新颖的被修改的基础和甲基化的核糖和主要发生在tRNA从极端嗜热的Crenarchaeota的古菌系统发育树。 2-硫代胸腺嘧啶存在于来自热球菌属的tRNA中，并且构成了古细菌RNA中唯一已知的胸腺嘧啶部分，与其在细菌和真核生物的tRNA中几乎无处不在的存在相反。 在本研究中，共有33个修饰的核苷在古细菌tRNA中进行了严格的表征，表明古细菌tRNA中转录后修饰的结构范围比以前已知的更广泛。 从系统发育的角度来看，某些tRNA修饰发生在古细菌中，这些修饰在细菌或真核生物中是独特的，尽管修饰的总体模式在真核生物中比在细菌中更典型。
Nucleoside modification has been studied in unfractionated tRNA from 11 thermophilic archaea (archaebacteria), including phylogenetically diverse representatives of thermophilic methanogens and sulfur-metabolizing hyperthermophiles which grow optimally in the temperature range of 56 (Thermoplasma acidophilum) to 105-degrees-C (Pyrodictium occultum), and for comparison from the most thermophilic bacterium (eubacterium) known, Thermotoga maritima (80-degrees-C). Nine nucleosides are found to be unique to the archaea, six of which are structurally novel in being modified both in the base and by methylation in ribose and occur primarily in tRNA from the extreme thermophiles in the Crenarchaeota of the archaeal phylogenetic tree. 2-Thiothymine occurs in tRNA from Thermococcus sp., and constitutes the only known occurrence of the thymine moiety in archaeal RNA, in contrast to its near-ubiquitous presence in tRNA from bacteria and eukarya. A total of 33 modified nucleosides are rigorously characterized in archaeal tRNA in the present study, demonstrating that the structural range of posttranscriptional modifications in archaeal tRNA is more extensive than previously known. From a phylogenetic standpoint, certain tRNA modifications occur in the archaea which are otherwise unique to either the bacterial or eukaryal domain, although the overall patterns of modification are more typical of eukaryotes than bacteria.