Evaluation of a test based on baculovirus-expressed glycoprotein G for detection of herpes simplex virus type-specific antibodies.
Evaluation of a test based on baculovirus-expressed glycoprotein G for detection of herpes simplex virus type-specific antibodies.
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基于杆状病毒表达的糖蛋白 G 的检测单纯疱疹病毒特异性抗体的评估。
DOI：
10.1093/infdis/164.6.1196
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Pellett,PE
中科院分区：
文献类型：
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作者：
Sánchez-Martínez,D;Schmid,DS;Whittington,W;Brown,D;Reeves,WC;Chatterjee,S;Whitley,RJ;Pellett,PE
使用表达HSV-1或-2糖蛋白G(gGI或gG2)的重组杆状病毒感染的昆虫细胞的提取物设计了用于区分针对1型和2型单纯疱疹病毒(HSV)的抗体的免疫印迹测定。通过将其结果与使用基于从HSV-1和HSV-2感染的细胞中免疫纯化的gG的免疫点测定获得的结果进行比较来评估该测定。 110份人血清标本均独立盲测3次。在血清稀释度为 1:20 时，gG1 和 gG2 的最大特异性分别为 96% 和 100%，最大灵敏度分别为 100% 和 92%。读数器的再现性为 99%，每个样本的单独测试样本的再现性为 95%。两个实验室从不同组的 15 个血清样本中获得的结果完全一致，表明该测定准确且可重复。抗原生产的简便性应该使该测试能够广泛应用。
An immunoblot assay for discrimination of antibodies to herpes simplex virus (HSV) types 1 and 2 was devised using extracts of recombinant-baculovirus-infected insect cells expressing HSV-l or -2 glycoprotein G (gGI or gG2). The assay was evaluated by comparing its results with those obtained by using an immunodot assay based on gG immunopurified from HSV-1- and HSV-2-infected cells. Each of 110 human serum specimens was tested blindly and independently three times. At a serum dilution of 1:20, the maximum specificities were 96% and 100% and the maximum sensitivities were 100% and 92% for gG1 and gG2, respectively. Reproducibility was 99% among readers and 95% among individually tested samples of each specimen. Results obtained in two laboratories from a different set of 15 serum specimens were in complete agreement, indicating the assay is accurate and reproducible. The ease of antigen production should allow the test to become widely available.