Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds
Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds
复制标题
外源抗坏血酸和谷胱甘肽引发对线粒体结构和功能系统的影响以减轻燕麦种子的老化损伤
DOI：
10.1186/s12870-020-2321-x
复制
发表时间：
2020-03-06
影响因子：
5.3
通讯作者：
Wang, Mingya
中科院分区：
文献类型：
--
作者：
Xia, Fangshan;Cheng, Hang;Wang, Mingya
在自然条件下，种子老化导致的活力丧失对农业生产产生不利影响。而灌浆是提高老化种子活力的一种经济有效的方法。本研究的目的是测试外源抗坏血酸（ASC）和谷胱甘肽（GSH）在老化燕麦种子修复中的有效性，并验证线粒体结构和功能系统参与这一过程的假设。结果将燕麦种子在45℃条件下人工陈化20 d，在20℃条件下分别用1 mmol L- 1的ASC、GSH或ASC + GSH溶液分别浸泡0.5 h。ASC、GSH和ASC + GSH后置处理显著提高了老化燕麦种子的种子萌发率、ASC-GSH循环中抗氧化酶、细胞色素c氧化酶（COX）和线粒体苹果酸脱氢酶（MDH）活性以及胚根细胞线粒体超微结构，丙二醛和H2O2含量显著降低（P < 0.05）。结论老化后分别灌注ASC、GSH或ASC + GSH均能有效缓解燕麦种子的老化损伤，且ASC的作用比GSH更有效，但ASC和GSH后灌注修复老化燕麦种子的积极作用并不优于ASC后灌注。然而，预浸ASC、GSH或ASC + GSH对燕麦种子没有效果，这表明预浸ASC、GSH或ASC + GSH不能抑制燕麦种子老化损伤的发生。
Background Loss of vigor caused by seed aging adversely affects agricultural production under natural conditions. However, priming is an economical and effective method for improving the vigor of aged seeds. The objective of this study was to test the effectiveness of exogenous ascorbic acid (ASC) and glutathione (GSH) priming in the repairing of aged oat (Avena sativa) seeds, and to test the hypothesis that structural and functional systems in mitochondria were involved in this process. Results Oat seeds were artificially aged for 20 days at 45 degrees C, and were primed with solutions (1 mmol L- 1) of ASC, GSH, or ASC + GSH at 20 degrees C for 0.5 h before or after their aging. Seed germination, antioxidant enzymes in the ASC-GSH cycle, cytochrome c oxidase (COX) and mitochondrial malate dehydrogenase (MDH) activities, and the mitochondrial ultrastructures of the embryonic root cells were markedly improved in aged oat seeds through post-priming with ASC, GSH, or ASC + GSH, while their malondialdehyde and H2O2 contents decreased significantly (P < 0.05). Conclusion Our results suggested that priming with ASC, GSH, or ASC + GSH after aging could effectively alleviate aging damage in oat seeds, and that the role of ASC was more effective than GSH, but positive effects of post-priming with ASC and GSH were not superior to post-priming with ASC in repairing aging damage of aged oat seeds. However, pre-priming with ASC, GSH, or ASC + GSH was not effective in oat seeds, suggesting that pre-priming with ASC, GSH, or ASC + GSH could not inhibit the occurrence of aging damage in oat seeds.