Aggregation-induced emission luminogens-encoded microspheres preparation and flow-through immunoaffinity chromatographic assay development for microcystin-LR analysis
Aggregation-induced emission luminogens-encoded microspheres preparation and flow-through immunoaffinity chromatographic assay development for microcystin-LR analysis
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DOI：
10.1016/j.foodchem.2022.134398
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发表时间：
2022-10-07
期刊：
影响因子：
8.8
通讯作者：
Jiang，Wenxiao
中科院分区：
文献类型：
--
作者：
Zhai，Peng;Liu，Chen;Jiang，Wenxiao
尽管经过数十年的努力，我们仍然面临着现场超痕量环境毒素检测的艰巨任务，特别是水华引起的微囊藻毒素。在这项工作中，设计了一种新型的基于荧光微球的流通免疫亲和色谱测定法，用于检测水和水产品中的超痕量微囊藻毒素-LR。将聚集诱导的发射发光体封装到荧光微球中，以确保微囊藻毒素-LR 定量的整个分析时间小于 30 分钟。此外，还捕获了比色图像并进行了定量分析，其在水和水生肌肉样品中的检测限为 0.217 pg/mL，定量限为 0.362 pg/mL。所开发的免疫测定法的平均回收率范围为 79.1% 至 95.7%，相对标准偏差小于 13.4%。因此，经过验证的流通式免疫亲和色谱测定是一种易于使用的替代方案，可用于在皮克水平上现场筛查水和水生样品中的微囊藻毒素-LR。
Despite decades of efforts, we are faced with the daunting task of on-site ultratrace environmental toxins detecting, especially the microcystins caused by water bloom. In this work, a novel fluorescent microsphere-based flow-through immunoaffinity chromatographic assay has been designed for detecting ultratrace microcystin-LR in water and aquatic products. The aggregation-induced emission luminogens were encapsulated into fluorescent microspheres to ensure microcystin-LR quantitation with a whole analytical time of less than 30 min. Furthermore, the colorimetric images were captured and quantitatively analyzed, which offered a limit of detection at 0.217 pg/mL and a limit of quantitation at 0.362 pg/mL in water and aquatic muscle samples. The developed immunoassays provide average recovery ranging from 79.1 % to 95.7 %, with relative standard deviations less than 13.4 %. Thus, the validated flow-through immunoaffinity chromatographic assay is an easy-to-use alternative for on-site screening of microcystin-LR in water and aquatic samples at picogram levels.