Cultivation and identification of rat bone marrow-derived mesenchymal stem cells
Cultivation and identification of rat bone marrow-derived mesenchymal stem cells
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DOI：
10.3892/mmr.2014.2264
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发表时间：
2014-08-01
影响因子：
3.4
通讯作者：
Cao, Yingguang
中科院分区：
文献类型：
--
作者：
Song, Ke;Huang, Mengqi;Cao, Yingguang
骨髓间充质干细胞（BMSCs）具有形成多种间充质组织类型的潜能，是骨组织工程应用的细胞来源。本研究旨在建立一种简便有效的骨髓间充质干细胞培养方法。取大鼠股骨骨髓细胞进行体外培养，经体外传代筛选扩增骨髓间充质干细胞。此外，BMSCs的生物学特性进行了研究，特定的表面抗原的表达进行了评估，使用流式细胞术和多能分化潜能的特点，证明使用标准的体外条件。获得单鳃异质细胞。在第3代，总共98.4%的细胞表达分化簇（CD）29和CD 90，但不表达CD 45。细胞能够分化为成骨细胞和成脂细胞。结论：从大鼠骨髓中分离的具有上述特性的骨髓间充质干细胞可作为骨组织工程的种子细胞。
Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to form a variety of mesenchymal tissue types, which are a source of cells for bone tissue engineering applications. The present study attempted to establish an effective and convenient method for culturing BMSCs. Total bone marrow cells, which were harvested from rat femurs, were cultured and BMSCs were selected and expanded through passaging in vitro. Furthermore, the biological properties of BMSCs were investigated, specific surface antigen expression was assessed using flow cytometry and the multipotent differentiation potential characteristics were demonstrated using standard in vitro conditions. Monoptychial heterogeneous cells were obtained. A total of 98.4% of cells at passage 3 expressed cluster of differentiation (CD)29 and CD90, but not CD45. The cells were able to differentiate into osteogenic and adipogenic cells. In conclusion, BMSCs that are isolated from the rat bone marrow and exhibit the identified characteristics may be used as seed cells in bone tissue engineering.