Responses of photosynthesis and phosphorylation of the light-harvesting complex of photosystem II to chilling temperature in ecologically divergent cultivars of rice
Responses of photosynthesis and phosphorylation of the light-harvesting complex of photosystem II to chilling temperature in ecologically divergent cultivars of rice
复制标题
DOI：
10.1016/s0098-8472(99)00008-8
复制
发表时间：
1999-06-01
影响因子：
5.7
通讯作者：
Heilman, JL
中科院分区：
文献类型：
--
作者：
Gesch, RW;Heilman, JL
水稻（Oryza sativa L.），其易受寒冷损害，在从热带到温带区域的各种环境中栽培。在本研究中，光合作用对长期低温的反应进行了评估，在其生态起源不同的五个水稻品种。此外，该假设进行了测试，当冷却超过几天，适应温带气候的水稻可能会保持更大的能力磷酸化的捕光蛋白复合物的PSII（LHCII）比水稻培育的热带环境。幼苗在28/22 ℃昼夜温度和550 μ mol m（-2）s（-1）的PPFD下生长，直到第三片叶完全展开，此时将它们转移到10/8 ℃昼夜温度和相同的光照强度下6 d，然后分别在18/15 ℃和25/21 ℃下恢复1 d和2 d。叶片光合作用下降了40-89%，在3天内转移到10/8摄氏度的品种研究，与显着差异发展在2天内。PSII的光化学效率，通过F-V/F-M测量。也有类似的趋势。植物复温后，光合作用和光化学完全恢复在两个温带粳稻品种研究，而只有部分恢复为籼稻和热带粳稻品种。长期低温导致温带粳稻LHCII磷酸化能力略有降低，而热带粳稻则抑制了45%。结果表明，不同生态类型水稻品种的CO2同化速率和光化学效率对低温的响应存在显著差异，低温恢复速率也存在显著差异。此外，维持和/或发展对LHCII和其他与PSII相关的类囊体蛋白磷酸化能力丧失的抗性可能有助于防止光损伤。(C)1999 Elsevier Science B. V.保留所有权利。
Rice (Oryza sativa L.), which is susceptible to chilling damage, is cultivated in diverse environments ranging from tropical to temperate regions. In the present study photosynthetic responses to long-term chilling were evaluated for five rice cultivars differing in their ecological origins. Furthermore, the hypothesis was tested that when chilled over several days, rice adapted to temperate climates may maintain a greater capacity for phosphorylation of the light-harvesting protein complex of PSII (LHCII) than rice bred for tropical environments. Seedlings were grown at 28/22 degrees C day/night temperature and PPFD of 550 mu mol m(-2) s(-1) until the third leaf was fully expanded, at which time they were transferred to 10/8 degrees C day/night temperature under the same light intensity for 6 d, followed by recovery at 18/15 degrees C and 25/21 degrees C, for 1 and 2 d, respectively. Leaf photosynthesis decreased by 40-89% within 3 d after transfer to 10/8 degrees C for the cultivars studied, with significant differences developing within 2 d. Photochemical efficiency of PSII, measured by F-V/F-M. followed a similar trend. After rewarming plants, photosynthesis and photochemistry fully recovered in two temperate Japonica cultivars studied, while only partial recovery was observed for Indica and tropical Japonica cultivars. Long-term chilling led to only a slight reduction in the capacity for LHCII phosphorylation in a temperate Japonica rice, while it was inhibited by 45% in a tropical Japonica type. The results indicate that the responses of CO2 assimilation rate and photochemical efficiency significantly vary among ecologically divergent cultivars of rice to chilling, as does their rate of recovery from chilling. Additionally, maintenance and/or development of resistance to the loss in capacity for the phosphorylation of LHCII, and other thylakoid proteins associated with PSII, may help to prevent photodamage. (C) 1999 Elsevier Science B.V. All rights reserved.