Nodamura virus nonstructural protein B2 can enhance viral RNA accumulation in both mammalian and insect cells
Nodamura virus nonstructural protein B2 can enhance viral RNA accumulation in both mammalian and insect cells
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DOI：
10.1128/jvi.78.12.6698-6704.2004
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发表时间：
2004-06-01
影响因子：
5.4
通讯作者：
Ball, LA
中科院分区：
文献类型：
--
作者：
Johnson, KL;Price, BD;Ball, LA
在脊椎动物和无脊椎动物细胞系的感染过程中，甲型新村病毒从B2开放阅读框表达两种不同长度的非结构蛋白。这些蛋白质的功能尚未确定，但相关的鸡舍病毒B2在果蝇细胞和转基因植物中都能抑制RNA干扰。为了验证Nov B2蛋白是否具有相似的功能，我们比较了野生型Nov RNA的复制情况和不能制造B2蛋白的突变体的复制情况。我们观察到突变病毒RNA的积累存在缺陷，在某些细胞系(例如，小仓鼠肾细胞)中可以忽略不计，在其他细胞系(例如，人HeLa和果蝇DL-1细胞)中存在严重缺陷。这些结果与NOVB2蛋白绕过先天抗病毒反应的概念是一致的，例如在不同宿主细胞之间效力不同的RNA干扰。
During infection of both vertebrate and invertebrate cell lines, the alphanodavirus Nodamura virus (NoV) expresses two nonstructural proteins of different lengths from the B2 open reading frame. The functions of these proteins have yet to be determined, but B2 of the related Flock House virus suppresses RNA interference both in Drosophila cells and in transgenic plants. To examine whether the NoV B2 proteins had similar functions, we compared the replication of wild-type NoV RNA with that of mutants unable to make the B2 proteins. We observed a defect in the accumulation of mutant viral RNA that varied in extent from negligible in some cell lines (e.g., baby hamster kidney cells) to severe in others (e.g., human HeLa and Drosophila DL-1 cells). These results are consistent with the notion that the NoV B2 proteins act to circumvent an innate antiviral response such as RNA interference that differs in efficacy among different host cells.