Cardiac expression of polysialylated NCAM in the chicken embryo: correlation with the ventricular conduction system.
Cardiac expression of polysialylated NCAM in the chicken embryo: correlation with the ventricular conduction system.
复制标题
鸡胚中多唾液酸化 NCAM 的心脏表达：与心室传导系统的相关性。
DOI：
10.1002/aja.1001940206
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Fallah-Najmabadi,H
中科院分区：
文献类型：
--
作者：
Watanabe,M;Timm,M;Fallah-Najmabadi,H
神经细胞粘附分子（NCAM）及其多聚唾液酸部分（PSA）在神经系统和骨骼肌发育过程中影响细胞间的相互作用。NCAM还在包括人类在内的各种物种的胚胎心脏中被鉴定。然而，关于NCAM及其功能调节PSA在心脏发生中的作用的知识有限。用间接免疫荧光染色法研究了鸡胚心室肌中NCAM及其PSA的分布。NCAM多肽被发现在整个心肌。与此相反，PSA位于第20至44期胚胎的离散区域（在分隔期间和之后）。到第20期时，房室管和心室小梁内膜下区域的肌纤维PSA呈阳性。在后期，房室交界面正下方的间隔后心脏横切面显示间隔内有PSA阳性心肌束。该束在更顶部水平连续有两个分支，这两个分支又与PSA阳性的左心室和右心室内膜下心肌连续。心肌中PSA的这种模式与成人心脏中定义的心室传导系统构型相似。室间隔内膜下的电子显微镜照片显示含肌原纤维的细胞上PSA阳性，浦肯野纤维的超微结构位置。在后期（35-44），PSA阳性区域内的一个细胞亚群被成人浦肯野纤维中发现的肌球蛋白重链同种型抗体染色。这些细胞和周围组织在成人心脏中缺乏PSA。因此，聚唾液酸化的NCAM可能在心室传导系统的发育过程中调节细胞-细胞相互作用。© 1992 Wiley利斯公司
The neural cell adhesion molecule (NCAM) and its polysialic acid moeity (PSA) affect cellular interactions during the development of the nervous system and skeletal muscle. NCAM has also been identified in the embryonic heart of various species including humans. However, knowledge regarding the role of NCAM and its function‐modulating PSA in cardiogenesis is limited. The distribution of NCAM and its PSA in the ventricular myocardium of chicken embryos was determined by indirect immunofluorescence staining. The NCAM polypeptide was found throughout the cardiac myocardium. In contrast PSA was located in discrete regions in stage 20 to 44 embryos (during and after septation). Myocardium at the subendocardial regions of the atrioventricular canal and ventricular trabeculae were PSA positive by stage 20. At later stages, transverse sections of the postseptation heart just below the level of the atrioventricular interface revealed a PSA‐positive bundle of myocardium in the septum. This bundle was continuous with two branches at a more apicallevel which in turn were continuous with the PSA‐positive subendocardial myocardium lining the left and right ventricles. This pattern of PSA in the myocardium was similar to that of the ventricular conduction system configuration defined in the adult heart. Electron micrographs of the subendocardium of the ventricular septum revealed PSA positivity on myofibril‐containing cells with the ultrastructural location of Purkinje fibers. At later stages (35–44) a subset of cells within PSA‐positive regions was stained by an antibody against an isoform of the myosin heavy chain found in adult Purkinje fibers. These cells and surrounding tissue lacked PSA in the adult heart. Thus polysialylated NCAM may be modulating cell–cell interactions during the development of the ventricular conduction system. © 1992 Wiley‐Liss, Inc.