Physiological and biochemical responses of transgenic potato plants with altered expression of PSII manganese stabilizing protein
Physiological and biochemical responses of transgenic potato plants with altered expression of PSII manganese stabilizing protein
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DOI：
10.1016/j.plaphy.2012.07.003
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发表时间：
2012-09-01
影响因子：
6.5
通讯作者：
Park, Se Won
中科院分区：
文献类型：
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作者：
Gururani, Mayank Anand;Upadhyaya, Chandrama Prakash;Park, Se Won
锰稳定蛋白（MSP）是放氧复合物（OEC）的重要组成部分。产生具有增强的（正义）和降低的（反义）MSP表达水平的转基因马铃薯植物，以研究MSP在整体植物生长，特别是在块茎发育中的可能生理作用。MSP反义植株表现出较高的结薯频率和较高的块茎产量，增加总可溶性碳水化合物。使用OJIP动力学检查植物的光合效率; MSP反义植物的光合作用比MSP正义和UT（未转化的）对照植物更活跃。氧测量表明相对氧释放与MSP表达成正比，因为MSP反义植物与MSP正义植物以及UT植物相比显示出低得多的氧释放。MSP-感生植物在形态、块茎产量和光合性能方面表现得与UT植物相似。叶绿素a荧光分析表明MSP反义植物中可能缺乏完整的释氧复合物（OEC），其允许接近内部非水电子供体（例如，抗坏血酸和脯氨酸），并因此增加那些植物的光系统II（PSII）活性。这些发现进一步表明，这种改变的光合机制可能与早期结薯和增加结薯频率。(C)2012年Elsevier Masson SAS。All rights reserved.
Manganese-stabilizing protein (MSP) represents a key component of the oxygen-evolving complex (OEC). Transgenic potato plants with both enhanced (sense) and reduced (anti-sense) MSP expression levels were generated to investigate the possible physiological role of MSP in overall plant growth, particularly in tuber development. MSP antisense plants exhibited both higher tuberization frequency and higher tuber yield with increased total soluble carbohydrates. The photosynthetic efficiencies of the plants were examined using the OJIP kinetics; MSP-antisense plants were photosynthetically more active than the MSP-sense and UT (untransformed) control plants. The oxygen measurements indicated that the relative oxygen evolution was directly proportional to the MSP expression, as MSP-antisense plants showed much lower oxygen evolution compared to MSP-sense as well as UT plants. MSP-sense plants behaved like the UT plants with respect to morphology, tuber yield, and photosynthetic performance. Chlorophyll a fluorescence analyses indicate a possible lack of intact Oxygen Evolving Complexes (OECs) in MSP antisense plants, which allow access to internal non-water electron donors (e.g., ascorbate and proline) and consequently increase the Photosystem II (PSII) activity of those plants. These findings further indicate that this altered photosynthetic machinery may be associated with early tuberization and increased tuberization frequency. (C) 2012 Elsevier Masson SAS. All rights reserved.