Circulating cell-free DNA in plasma of colorectal cancer patients - A potential biomarker for tumor burden
Circulating cell-free DNA in plasma of colorectal cancer patients - A potential biomarker for tumor burden
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DOI：
10.1016/j.suronc.2017.08.001
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发表时间：
2017-12-01
影响因子：
2.3
通讯作者：
Mannhalter, Christine
中科院分区：
文献类型：
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作者：
Bhangu, Jagdeep Singh;Taghizadeh, Hossein;Mannhalter, Christine
背景：血浆中游离DNA（cfDNA）的测量-所谓的液体活检-是早期检测癌症的一种新方法。坏死的癌细胞释放出各种DNA片段，这些片段可以在血浆或血清中检测到。我们研究的目的是调查来自结直肠癌（CRC）患者的静脉和动脉血的血浆中的循环LINE 115、LINE 79和LINE 297片段在手术干预之前、期间和之后5天的浓度。本研究纳入了30例接受结直肠癌和阑尾癌手术的患者（16例女性，14例男性，中位年龄56岁）和17例健康志愿者。从患者和健康个体收集血浆样品。用特异性引物对大肠癌患者血浆中的大肠癌115、LINE 79和LINE 297进行定性PCR和实时荧光定量PCR分析。结果：大肠癌患者血浆中的大肠癌115浓度显著高于对照组（p = 0.002）。有趣的是，LINE 297在健康个体中的浓度显著高于患者（p = 0.031）。我们未发现两组之间关于LINE 79的任何差异（p = 0.893）。手术后血浆中的总cfDNA浓度略微增加（p < 0.056），然而，差异不显著。结论：cfDNA中的p53 115浓度可能是诊断结直肠癌的一个潜在的生物标志物。LINE 79或常规肿瘤标志物CEA或CA 19 -9似乎对恶性肿瘤的检测没有用。cfDNA中LINE 297的量是否代表早期诊断的可靠生物标志物尚未得到证实。(C)2017爱思唯尔有限公司版权所有
Background: Measurement of cell-free DNA (cfDNA) in plasma - the so called liquid biopsy - is a novel method for early detection of cancer. Necrotic cancer cells release various DNA fragments that can be detected in plasma or serum. The aim of our study was to investigate the concentration of circulating ALU115, LINE79 and LINE297 fragments in plasma from venous and arterial blood of colorectal cancer (CRC) patients before, during and 5 days after surgical intervention.Patients and methods: Thirty patients (16 female, 14 male, median age 56 years), undergoing surgery for colorectal and appendix cancer, and 17 healthy volunteers were included in this study. Plasma samples were collected from patients and healthy individuals. Qualitative polymerase chain reaction (PCR) and quantitative real-time PCR analyses were conducted using specific primers for ALU115, LINE79 and LINE297.Results: The concentration of ALU115 was significantly increased in plasma of CRC patients compared to the control group (p = 0.002). Interestingly, the concentration of LINE297 was significantly higher in healthy individuals than patients (p = 0.031). We did not find any difference regarding LINE79 between the two groups (p = 0.893). The total cfDNA concentration was slightly increased in plasma after the surgery (p < 0.056), however, the difference was not significant. Interestingly, no correlation was detected between the peritoneal carcinosis index (PCI) and conventional tumor markers.Conclusion: According to our results, the concentration of ALU115 in cfDNA could be a potential biomarker for diagnosis of CRC. LINE79 or the conventional tumor markers CEA or CA19-9 do not seem useful for the detection of malignant tumors. Whether the amount of LINE297 in cfDNA represents a reliable biomarker for early diagnosis has yet to be confirmed. (C) 2017 Elsevier Ltd. All rights reserved.