Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats
Inactivation of the Ventral Pallidum by GABAA Receptor Agonist Promotes Non-rapid Eye Movement Sleep in Rats
复制标题
GABA(A) 受体激动剂灭活腹侧苍白球促进大鼠非快速眼动睡眠
DOI：
10.1007/s11064-020-03040-z
复制
发表时间：
2020-05-04
影响因子：
4.4
通讯作者：
Xu, Haibo
中科院分区：
文献类型：
--
作者：
Zhang, Xuefen;Liu, Ying;Xu, Haibo
GABA是大脑中最丰富的抑制性神经递质，与睡眠和觉醒密切相关。腹侧苍白球（ventral pallidum，VP）的最大输入区域--中脑腹侧核（nucleus nucleus，NAc）通过抑制表达NAc腺苷A（adenosine A，2A）受体的神经元向主要表达GABA（A）受体的VP GABA能神经元的投射，在促进非快动眼睡眠（non-rapid eye movement，NREM）中发挥重要作用。虽然这些研究证实了VP GABA能神经元在睡眠-觉醒调节中的可能作用，但其是否以及如何调节睡眠-觉醒周期尚不完全清楚。本研究通过对自由活动的大鼠进行药物干预，记录大鼠的脑电图和肌电图，监测大鼠的睡眠-觉醒状态。我们发现，在VP内微量注射GABA（A）受体激动剂蝇蕈醇，在明期和暗期都增加了NREM睡眠。VP内微量注射GABA（A）受体拮抗剂荷包牡丹碱可增加光照期的觉醒。总之，我们的数据确定了VP GABA（A）受体表达神经元在大鼠NREM睡眠中的重要作用，这可能有助于提高对病理性睡眠障碍的理解。
GABA, the most abundant inhibitory neurotransmitter in the brain, is closely linked with sleep and wakefulness. As the largest area input to the ventral pallidum (VP), the nucleus accumbens (NAc) has been confirmed to play a pivotal role in promoting non-rapid eye movement (NREM) sleep through inhibitory projections from NAc adenosine A(2A) receptor-expressing neurons to VP GABAergic neurons which mostly express GABA(A) receptors. Although these studies demonstrate the possible role of VP GABAergic neurons in sleep-wake regulation, whether and how its modulate sleep-wake cycle is not completely clear. In our study, pharmacological manipulations were implemented in freely moving rats and then the EEG and the EMG were recorded to monitor the sleep-wake states. We found that microinjection of muscimol, a GABA(A) receptor agonist, into the VP increased NREM sleep in both light and dark period. Microinjection of bicuculline, a GABA(A) receptor antagonist, into the VP increased wakefulness in the light period. Collectively, our data identify the important role of VP GABA(A) receptor-expressing neurons in NREM sleep of rats which may help improve the understanding of the pathological sleep disorders.