High-Fat Diet Causes Constipation in Mice via Decreasing Colonic Mucus
High-Fat Diet Causes Constipation in Mice via Decreasing Colonic Mucus
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DOI：
10.1007/s10620-019-05954-3
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发表时间：
2020-08-01
影响因子：
3.1
通讯作者：
Itoh, Yoshito
中科院分区：
文献类型：
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作者：
Mukai, Rieko;Handa, Osamu;Itoh, Yoshito
背景便秘是最常见的胃肠道疾病之一。虽然便秘的原因多种多样，但饮食习惯影响很大。过多的脂肪摄入被认为是便秘的主要原因之一；然而，确切的机制尚不清楚。目的 研究高脂肪饮食 (HFD) 是否会导致小鼠便秘，并阐明其潜在机制，重点关注结肠粘液量。方法将6周龄雄性C57BL/6小鼠随机分为两组：HFD喂养组和正常饲料（NCD）喂养组。测量粪便重量、含水量、总胃肠道通过时间和结肠通过时间以确定小鼠是否便秘。通过免疫染色评估结肠粘液并通过光谱测定法定量。使用硫代巴比妥酸（TBA）测试测量丙二醛（MDA）作为氧化应激的标志物。结果与NCD组相比，HFD组粪便重量较轻。在功能实验中，HFD组的总胃肠道通过时间和结肠通过时间更长。此外，HFD 显着减少了结肠粘液的量。此外，表面活性剂引起的结肠粘液减少导致非传染性疾病组出现便秘。结论 HFD 导致小鼠便秘并延迟结肠传输时间，可能是通过减少小鼠结肠粘液来实现的。
Background Constipation is one of the most common gastrointestinal complaints. Although the causes of constipation are varied, dietary habits have a significant influence. Excessive fat intake is suggested as one of the main causes of constipation; however, the exact mechanism is unknown. Aims To investigate whether a high-fat diet (HFD) causes constipation in mice and to clarify the underlying mechanism, focusing on the amount of colonic mucus. Methods Six-week-old male C57BL/6 mice were randomly divided into two groups: mice fed with HFD and those with normal chow diet (NCD). Fecal weight, water content, total gastrointestinal transit time, and colon transit time were measured to determine whether the mice were constipated. The colonic mucus was evaluated by immunostaining and quantified by spectrometry. Malondialdehyde (MDA) was measured using the thiobarbituric acid (TBA) test as a marker for oxidative stress. Results Compared to the NCD group, the weight of feces was less in the HFD group. In the functional experiment, the total gastrointestinal transit time and colon transit time were longer in the HFD group. Furthermore, HFD significantly reduced the amount of colonic mucus. In addition, the reduction in colonic mucus caused by surfactant resulted in constipation in the NCD group. Conclusions HFD causes constipation with delayed colon transit time possibly via the reduction in colonic mucus in mice.