Dendritic spine changes associated with hippocampal long-term synaptic plasticity
Dendritic spine changes associated with hippocampal long-term synaptic plasticity
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DOI：
10.1038/19978
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发表时间：
1999-05-06
期刊：
影响因子：
64.8
通讯作者：
Bonhoeffer, T
中科院分区：
文献类型：
--
作者：
Engert, F;Bonhoeffer, T
海马体中突触效能的长期增强是研究神经元可塑性、回路重组，乃至学习与记忆的细胞机制的一个重要模型。尽管这些持久的功能变化很容易诱导，但要证明它们伴有甚至是由亚细胞水平的形态变化所引起却非常困难。在此，我们将局部灌流技术与双光子成像技术相结合，这使我们能够仔细观察我们已知必然会发生突触变化的突触后树突的特定区域。我们表明，在CA1区诱导突触的持久（而非短暂）功能增强后，突触后树突上会出现新的棘突，而在同一树突的对照区域或长时程增强被阻断的脑片中，没有出现明显的棘突生长。
Long-term enhancement of synaptic efficacy in the hippocampus is an important model for studying the cellular mechanisms of neuronal plasticity, circuit reorganization, and even learning and memory(1). Although these long-lasting functional changes are easy to induce, it has been very difficult to demonstrate that they are accompanied or even caused by morphological changes on the subcellular level Here we combined a local superfusion technique(2,3) with two-photon imaging(4), which allowed us to scrutinize specific regions of the postsynaptic dendrite where we knew that the synaptic changes had to occur. We show that after induction of long-lasting (but not short-lasting) functional enhancement of synapses in area CAI, new spines appear on the postsynaptic dendrite, whereas in control regions on the same dendrite or in slices where long-term potentiation was blocked, no significant spine growth occurred.