Multiplication of Dutch Iris (Iris hollandica) by Organ Culture
Multiplication of Dutch Iris (Iris hollandica) by Organ Culture
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通过器官培养繁殖荷兰鸢尾 (Iris hollandica)
DOI：
10.2503/jjshs.41.66
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发表时间：
1972
影响因子：
--
通讯作者：
Kunihiko Hamada
中科院分区：
文献类型：
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作者：
M. Fujino;Tadashi Fujimura;Kunihiko Hamada
以荷兰鸢尾（Iris hollandica cv.韦奇伍德在改良的Kano、Neergarrd和Morel培养基上进行芽的离体培养。附加NAA和BA的培养基能诱导外植体不定芽的形成，但根的形成受到抑制。在仅含有NAA作为植物生长调节剂的培养基上观察到根的形成。高浓度的无机盐有利于外植体的生长，以羊肚菌和改良卡诺培养基效果较好。在改良Kano培养基中添加蛋白胨，2g/l及更高浓度的蛋白胨抑制外植体的生长。pH5.0和6.0时外植体生长良好。琼脂和蔗糖的最适浓度分别为2g/l和2- 4%。
Organ formation has been studied with lateral buds excised from bulbs of Iris hollandica cv.‘Wedgwood.’The buds were cultured in vitro on the medium of modified Kano, Neergarrd and Morel. Adventitious bud formation of explants was induced on the medium containing both NAA and BA, but root formation was suppressed. Root formation was observed on the medium containing NAA alone as a plant growth regulator. High concentration of mineral salt favoured the growth of explants, and better results were obtained on the medium based on Morel or modified Kano. Peptone added into modified Kano′s medium inhibited the growth of explants at 2g/l and higher concentrations. The growth of explants was good at pH 5.0 and 6.0. The optimal concentrations for agar and sucrose were 2g/l and 2-4%, respectively.