Cell adhesion behaviors on spider silk fibers, films, and nanofibers
Cell adhesion behaviors on spider silk fibers, films, and nanofibers
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蜘蛛丝纤维、薄膜和纳米纤维上的细胞粘附行为
DOI：
10.1021/acs.langmuir.2c00818
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发表时间：
2022
期刊：
影响因子：
3.9
通讯作者：
Jun Negishi
中科院分区：
文献类型：
--
作者：
Kenjiro Yazawa;Kosuke Hidaka;Jun Negishi
丝基材料由于其机械韧性和低细胞毒性而引起了人们的关注，被用作医疗用品。蚕丝作为外科缝合线已有几十年的历史。相比之下，蜘蛛丝的利用受到限制，主要是因为它的稀缺性。虽然利用基因工程技术利用重组蛋白表达系统开发了蜘蛛丝的仿生学，但产品通常分子量较低且缺乏N端或c端区域。蜘蛛丝样蛋白的不完整序列阻碍了对天然蜘蛛丝作为医学应用的客观评价，并阻碍了蜘蛛丝启发材料的发展。在此，我们直接从活蜘蛛身上卷取天然蜘蛛丝，并基于纤维、薄膜和无纺布三种蜘蛛丝基基质的表面形貌，研究了细胞的粘附行为。细胞的粘附行为主要受表面微纳结构的影响，而非表面的润湿性。该研究将有助于促进蜘蛛丝作为一种有前途的生物基纤维在可持续发展目标背景下在医学领域的利用。
Silk-based materials have garnered attention for use as medical supplies due to their mechanical toughness and low cytotoxicity. Silkworm silk has been applied as surgical sutures for decades. In contrast, the utilization of spider silk is limited mainly because of its scarcity. Although the biomimicry of spider silk has been developed using recombinant protein expression systems with the use of genetic engineering, the product often results in lower molecular weight and a lack of the N- or C-terminal regions. The incomplete sequence of the spider silk-like protein prevents the objective evaluation of the native spider silk as a medical application and retards the development of spider silk-inspired materials. Here, we reeled the native spider silk directly from live spiders and investigated the cell adhesion behavior based on three kinds of surface topography of spider silk-based substrates, namely, fibers, films, and non-woven fabrics. The cell adhesion behavior was largely influenced by the surface micro/nanostructure rather than the wettability of the surface. This study will contribute to promote the utilization of spider silk in the medical field as a candidate for promising bio-based fibers in the context of sustainable development goals.