Fibrolamellar carcinomas show overexpression of genes in the RAS, MAPK, PIK3, and xenobiotic degradation pathways
Fibrolamellar carcinomas show overexpression of genes in the RAS, MAPK, PIK3, and xenobiotic degradation pathways
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DOI：
10.1016/j.humpath.2006.07.019
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发表时间：
2007-04-01
期刊：
影响因子：
3.3
通讯作者：
Torbenson, Michael
中科院分区：
文献类型：
--
作者：
Kannangai, Rajesh;Vivekanandan, Perumal;Torbenson, Michael
纤维板层癌（FLC）是一种罕见的原发性肝细胞癌，见于年轻人。已知FLC具有相对较少的一致的染色体改变，尽管已经报道了染色体1 q的增加。使用Affyscore DNA微阵列技术（Santa Clara，CA）研究4种FLC（2种原发性FLC和2种转移性沉积物）的基因表达。通过实时聚合酶链反应确认所选基因。相对较少的基因显著过表达-447个基因，情况1; 1298个基因，情况2-分别对应于阵列中存在的56000个转录本的约0.8%和2.3%。其中，155个基因在两种肿瘤中同时过表达。在2个转移性沉积物中显著过表达的基因的数量增加了一倍以上（2777和2855个基因，而原发性肿瘤中为1298个基因）。参与RAS、MAPK、PIK 3和异生物质降解途径的蛋白质通常过表达。由于染色体1 q被认为包含一个重要的致癌基因，额外的注意力集中在这个区域。在所有原发性和转移性肿瘤中共同发现过表达的总共114个基因中，114个基因中的111个位于染色体1 q上：ARF 1、CD 46、CNIH 4、ENSA、FH、NICE-3、PSMB 4、RGS 2、RGS 5、TIMM 17 A和UFC 1。原发性FLC显示参与RAS、MAPK、PIK 3和异生物质降解途径的基因过表达。在所有肿瘤和转移瘤中，11个共同基因在染色体1 q上持续过表达，并作为潜在的癌基因值得进一步研究。(c)2007年爱思唯尔公司All rights reserved.
Fibrolamellar carcinomas (FLC) are a rare type of primary hepatocellular carcinoma found in Younger individuals. FLC are known to have relatively few consistent chromosomal alterations, although a gain of chromosome 1q has been reported. The gene expression of 4 FLC (2 primary FLC and 2 metastatic deposits) were studied using Affymetrix DNA microarray technology (Santa Clara, CA). Selected genes were confirmed by real-time polymerase chain reaction. Relatively few genes were significantly overexpressed-447 genes, case 1; 1298 genes, case 2-corresponding to approximately 0.8% and 2.3%, respectively, of the 56 000 transcripts present in the arrays. Of these, 155 genes were overexpressed simultaneously by both tumors. The number of significantly overexpressed genes more than doubled in the 2 metastatic deposits (2777 and 2855 genes compared with 1298 in the primary tumor). Proteins involved in the RAS, MAPK, PIK3, and xenobiotic degradation pathways were commonly overexpressed. Because chromosome 1q is thought to contain an important oncogene, additional attention was focused on this region. Of 114 total genes found overexpressed in common among all primary and metastatic tumors, I I of 114 genes were located on chromosome 1q: ARF1, CD46, CNIH4, ENSA, FH, NICE-3, PSMB4, RGS2, RGS5, TIMM17A, and UFC1. Primary FLC show overexpression of genes involved in the RAS, MAPK, PIK3, and xenobiotic degradation pathways. Eleven common genes were consistently overexpressed on chromosome 1q among all tumors and metastases and warrant further study as potential oncogenes. (c) 2007 Elsevier Inc. All rights reserved.