Phosphoserine – a convenient compound for modification of calcium phosphate bone cement collagen composites
Phosphoserine – a convenient compound for modification of calcium phosphate bone cement collagen composites
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DOI：
10.1023/b:jmsm.0000021119.14870.3d
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发表时间：
2004-04
期刊：
影响因子：
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通讯作者：
A. Reinstorf;M. Ruhnow;M. Gelinsky;W. Pompe;U. Hempel;K. Wenzel;P. Simon
中科院分区：
文献类型：
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作者：
A. Reinstorf;M. Ruhnow;M. Gelinsky;W. Pompe;U. Hempel;K. Wenzel;P. Simon
基于磷酸钙和羟基磷灰石（HAP）的临时骨替代材料用于外科手术中填充骨缺损。能够通过其官能团控制HAP的成核和晶体生长并且可以另外激活骨细胞的组分可能有助于开发具有增强的体内重塑的材料。本文研究了O-磷酸-L-丝氨酸（PS）对磷酸钙骨水泥复合材料性能的影响。对于高达25 mg/g PS的添加量，测定了水泥在负荷下的稳定性的强烈增加。用扫描电子显微镜和透射电子显微镜对材料进行了研究。与未改性的样品相比，在改性的复合材料中检测到更致密的微观结构和板状形态的HAP晶体。X射线粉末衍射分析表明，α-TCP和DCPA颗粒的溶解受到抑制，α-TCP和DCPA是水泥前驱体的主要成分。使用大鼠颅骨成骨细胞的细胞培养研究的结果表明，PS改性材料上的细胞具有良好的活力。此外，PS修饰的材料上的增殖和分化被发现是增强。
Temporary bone replacement materials on the basis of calcium phosphates and hydroxyapatite (HAP) are used in surgery for filling bone defects. Components which are able to control the nucleation and crystal growth of HAP through their functional groups and which can additionally activate bone cells may be helpful in the development of materials with enhanced remodelling in vivo. In this study, the influence of O-phospho-L-serine (PS) on the materials properties of calcium phosphate bone cement composites was investigated. For up to an addition of 25 mg/g PS a strong increase in the stability of the cements under load was determined. The material was studied by scanning electron microscopy and transmission electron microscopy. A more dense microstructure and a plate-like morphology of the HAP-crystals were detected in the modified composites compared with the non-modified samples. By X-ray powder diffraction an inhibition of the dissolution of α-tricalcium phosphate (α-TCP) and dicalciumphosphate anhydrous (DCPA) particles was found. α-TCP and DCPA are the main constituents of the cement precursor. The results of cell culture studies using rat calvaria osteoblasts demonstrate a good viability of the cells on the PS-modified material. Furthermore, the proliferation and differentiation were found to be enhanced on the PS-modified material.