Evaluation of dried blood spots as alternative sampling material for serological detection of anti-SARS-CoV-2 antibodies using established ELISAs
Evaluation of dried blood spots as alternative sampling material for serological detection of anti-SARS-CoV-2 antibodies using established ELISAs
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DOI：
10.1515/cclm-2020-1436
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发表时间：
2021-01-01
影响因子：
6.8
通讯作者：
Kern, Peter M.
中科院分区：
文献类型：
--
作者：
Weisser, Heike;Steinhagen, Katja;Kern, Peter M.
目的：在目前的大流行期间，基于静脉血清的抗体检测有助于确定受检者以前是否感染过SARS-CoV-2。或者，毛细血管血可以通过手指针刺（干血点，DBS）。在这项研究中，使用两种血清学检测方法对DBS和静脉穿刺样本进行检测，以评估DBS用于检测抗SARS-CoV-2抗体的可用性。从389名志愿者中采集了DBS和静脉血清的配对样本，其中75人最近有PCR证实的SARS-CoV-2感染，并使用两种ELISA检测针对病毒S1和核衣壳蛋白（NCP）抗原的抗SARS-CoV-2 IgG抗体。结果：DBS检测结果与S1法和NCP法检测结果基本一致，且相关性较高。结论：DBS检测结果与S1法和NCP法检测结果基本一致，且相关性较高。从DBS得到的ELISA结果显示与用血清得到的结果非常一致，假设DBS作为检测抗SARS-CoV-2抗体的样品材料具有足够的可用性和耐用性。近期将开展大规模的SARS-CoV-2抗体流行病学筛查。由于DBS减少了医疗机构在样本收集方面的压力，因此它们有可能在当前的SARS-CoV-2大流行中促进有效的社区和人群筛查。
Objectives: During the current pandemic, antibody testing based on venous serum helps to determine whether the tested person has been previously infected with SARS-CoV-2. Alternatively, capillary blood can be taken via a finger prick (dried blood spots, DBS). In this study, paired DBS and venipuncture samples were tested using two serological assays to evaluate the usability of DBS for the detection of anti-SARS-CoV-2 antibodies.Methods: Paired samples of DBS and venous serum were collected from 389 volunteers, of whom 75 had a recent PCR-confirmed SARS-CoV-2 infection, and tested for anti-SARS-CoV-2 IgG antibodies against both viral S1 and nucleocapsid protein (NCP) antigens using two ELISAs. Degree of agreement and correlation coefficients between ELISA results based on the two sampling methods were calculated.Results: Results of DBS showed almost perfect agreement and high correlations with results from corresponding serum samples in both the S1-based ELISA and the NCP-based ELISA.Conclusions: ELISA results derived from DBS showed very high agreement to those obtained with serum, supposing adequate usability and robustness of DBS as sample material for detection of anti-SARS-CoV-2 antibodies. In the near future, large-scale epidemiological screening for antibodies against SARS-CoV-2 will be carried out. Since DBS reduce the strain on healthcare institutions regarding sample collection, they have a potential to facilitate efficient community- and population-based screening in the current SARS-CoV-2 pandemic.