Design of infection-resistant antibiotic-releasing polymers: I. Fabrication and formulation
Design of infection-resistant antibiotic-releasing polymers: I. Fabrication and formulation
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DOI：
10.1016/s0168-3659(99)00106-6
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发表时间：
1999-12-06
影响因子：
10.8
通讯作者：
Ratner, BD
中科院分区：
文献类型：
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作者：
Kwok, CS;Wan, CX;Ratner, BD
假体植入物中经常观察到与生物材料相关的感染，并且在许多情况下会导致装置故障。为了设计一种本质上具有抗感染性的生物医学有用聚合物，我们开发了一种负载环丙沙星的聚氨酯（PU）基质，它可以在植入物表面局部释放抗生素，从而最大限度地减少细菌积累。我们在此报告了制造此类抗生素负载装置的制造和配方方法，以及其杀菌特性的证据。具体来说，检查了各种成孔剂和药物负载量。由 BIOSPAN(R) PU、聚乙二醇和环丙沙星组成的最佳配方可提供最长的缓释有效期（5 天）。释放的环丙沙星对铜绿假单胞菌（PA）的杀菌功效是不含抗生素的对照PU的四倍。这种杀菌效率是由于 PA 从表面脱离的增加。这些观察结果表明，释放的环丙沙星具有生物活性，可以防止细菌永久粘附在基质上，从而降低生物膜相关感染的可能性。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Biomaterials-related infections are often observed with prosthetic implants and in many cases result in the failure of the devices. To design a biomedically useful polymer that is intrinsically infection-resistant, we have developed a ciprofloxacin-loaded polyurethane (PU) matrix that releases antibiotic locally at the implant surface, thereby minimizing bacterial accumulation. We report here the methods of fabrication and formulation for making such antibiotic-loaded devices, as well as evidence of their bactericidal properties. Specifically, various pore-forming agents and drug loadings were examined. An optimum formulation consisting of BIOSPAN(R) PU, poly(ethylene glycol) and ciprofloxacin offered the longest effective period of sustained release (5 days). The bactericidal efficacy of the released ciprofloxacin against Pseudomonas aeruginosa (PA) was four times that of the control PU without antibiotics. This bactericidal efficiency was due to an increase in the PA detachment from the surface. These observations suggested that the released ciprofloxacin was biologically active in preventing the bacteria from permanently adhering to the substratum, and thus decreasing the possibility of biofilm-related infection. (C) 1999 Elsevier Science B.V. All rights reserved.