Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A
Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A
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DOI：
10.1186/s13028-014-0042-6
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发表时间：
2014-07-12
影响因子：
2.1
通讯作者：
Arzt, Jonathan
中科院分区：
文献类型：
--
作者：
Stenfeldt, Carolina;Pacheco, Juan M.;Arzt, Jonathan
心肌炎通常被认为是与口蹄疫病毒（FMDV）感染相关的死亡原因。然而，fmdv相关心肌炎的发病机制尚未详细描述。目前的报告描述了大量的FMDV与明显的单核炎症反应、间质水肿和心肌细胞变性有关，这两只猪在急性感染两种不同FMDV毒株中的任何一种时死亡。尽管临床进展相似，但两例心肌炎的形态学特征有明显差异，心肌炎症的强度也有显著差异。白细胞群的表型特征显示，在这两种情况下，炎症浸润主要由CD172a+、CD163+和CD44+细胞的组合组成，有CD8+细胞的明显亚群，但一直缺乏CD3+和CD21+细胞的检测。这表明，在两个观察到的病例中，fmdv相关的急性心肌炎症主要由单核细胞系的白细胞组成，具有明显的CD8+细胞群，基于序列切片中缺乏CD3检测，这些细胞可能代表NK细胞。
Myocarditis is often cited as the cause of fatalities associated with foot-and-mouth disease virus (FMDV) infection. However, the pathogenesis of FMDV-associated myocarditis has not been described in detail. The current report describes substantial quantities of FMDV in association with a marked mononuclear inflammatory reaction, interstitial edema and cardiomyocyte degeneration in the myocardium of two pigs that died during acute infection with either of two different strains of FMDV. Despite similar clinical progression, there was a marked variation in morphological characteristics of myocarditis with a significant difference in intensity of myocardial inflammation between the two cases. Phenotypic characterization of leukocyte populations revealed that in both cases, the inflammatory infiltrate consisted mainly of combinations of CD172a+, CD163+ and CD44+ cells, with a distinct subset of CD8+ cells, but with consistent lack of detection of CD3+ and CD21+ cells. This suggests that the FMDV-associated acute myocardial inflammation in the two observed cases consisted mainly of leukocytes of monocyte lineage, with a distinct population of CD8+ cells which, based on lack of CD3 detection in serial sections, are likely to represent NK cells.