Passive stretch modulates denervation induced alterations in skeletal muscle myosin heavy chain mRNA levels
Passive stretch modulates denervation induced alterations in skeletal muscle myosin heavy chain mRNA levels
复制标题
被动拉伸调节去神经诱导的骨骼肌肌球蛋白重链 mRNA 水平的改变
DOI：
10.1007/s004249900130
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Morgan
中科院分区：
文献类型：
--
作者：
P. Loughna;M. Morgan
抽象。去神经支配和去神经支配结合固定在缩短或延长的位置（被动拉伸）对肌球蛋白重链（MyHC）mRNA水平的影响进行了研究，在三个大鼠后肢肌肉具有不同的表型。去神经单独引起的I型和IIa型MyHC转录在所有三个肌肉的减少。与此相反，去神经引起了72%的增加，在IIb型的缓慢姿势比目鱼肌肌肉只有这是防止固定在延长的位置。在相同的肌肉被动拉伸也显着延迟失神经支配后的影响I型转录（从38%低于控制水平的24%以下）和IIa型转录（从59%至32%低于控制水平）。I型和IIa型转录物的水平，在快速相位足底肌肉，都不受拉伸结合去神经相比，单独去神经。在混合腓肠肌牵拉影响的I型，但不是IIa型转录水平。这些数据表明，被动拉伸可以独立于神经支配调节MyHC基因表达，但它是以肌肉特异性的方式进行的。
Abstract. The effect of denervation and denervation combined with immobilisation in either the shortened or lengthened position (passive stretch) upon myosin heavy chain (MyHC) mRNA levels was examined in three rat hind-limb muscles with differing phenotypes. Denervation alone caused a reduction in type I and type IIa MyHC transcripts in all three muscles. In contrast denervation caused a 72% increase in type IIb in the slow postural soleus muscle only which was prevented by immobilisation in the lengthened position. In the same muscle passive stretch also significantly retarded the effects of denervation upon the type I transcript (from 38% below control levels to 24% below) and type IIa transcript (from 59% to 32% below control levels). The levels of both type I and IIa transcripts, in the fast phasic plantaris muscle, were both unaffected by stretch combined with denervation when compared to denervation alone. In the mixed gastrocnemius muscle stretch affected the level of the type I but not the type IIa transcript. These data suggest that passive stretch can modulate MyHC gene expression independently of innervation but that it does so in a muscle-specific manner.
禽类慢肌球蛋白重链亚型表达的调节。
DOI：
10.1042/bj2600449
发表时间：
1989
期刊：
The Biochemical journal
影响因子：
--
作者：
Reid,SK;Kennedy,JM;Shimizu,N;Stewart,A;Vrbova,G;Zak,R
通讯作者：
Zak,R