Molecular diversity of foraminiferal eDNA in sediments and their correlations with environmental factors from the Yellow Sea
Molecular diversity of foraminiferal eDNA in sediments and their correlations with environmental factors from the Yellow Sea
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DOI：
10.1016/j.ecolind.2022.109294
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发表时间：
2022-08-15
影响因子：
6.9
通讯作者：
Li，Tiegang
中科院分区：
文献类型：
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作者：
Cao，Yifei;Lei，Yanli;Li，Tiegang
有孔虫环境DNA（eDNA）是环境变化的一种有效、可靠的指示剂。利用eDNA技术对黄海33-36°N 25个站位的有孔虫多样性进行了调查，首次对有孔虫多样性与环境条件的关系进行了评价。共采集了71个沉积物样本，并测量了当地环境参数。从样品中提取有孔虫eDNA，并从样品中扩增部分小亚基（SSU）rRNA基因。在高通量测序后，检测到1，701，899个扩增的读段，并聚类成426个操作分类单位（OTU）。底栖有孔虫eDNA群落多样性和类群多样性与水深和沉积物总无机碳（TIC）呈显著正相关，与温度和沉积物总有机碳（TOC）呈显著负相关。然而，在相对丰度与环境因子之间的相关性，有很大的变化，从物种到物种。在沉积物中检测到浮游有孔虫eDNA，其OTU数在一定程度上可以反映海流和上层水团的变化。这是西太平洋黄海陆架区有孔虫eDNA与环境因子关系的首次研究，表明有孔虫eDNA中的群落参数可以作为陆架海环境变化的一个有价值的指标。
Foraminiferal environmental DNA (eDNA) is an efficient and reliable indicator of environmental changes. Here, we investigated the foraminiferal diversity of 25 stations (33–36°N) in the Yellow Sea based on eDNA and presented the first assessment on the correlations between foraminifera diversity and environmental conditions in the region. A total of 71 sediment samples were collected and local environmental parameters were measured. The foraminiferal eDNA was extracted from the samples and part of the small subunit (SSU) rRNA gene was amplified from the samples. After high–throughput sequencing, 1,701,899 amplified reads were detected and clustered into 426 operational taxonomic units (OTUs). The benthic foraminiferal eDNA community diversity and group diversity showed significant positive correlations with water depth and total inorganic carbon (TIC) in sediments, and significant negative correlations with temperature and total organic carbon (TOC) in sediments. However, in terms of the correlation between relative abundance and environmental factors, there were wide variations from species to species. In this work, planktic foraminiferal eDNA was detected in the sediments, and its OTUs number could reflect the changes in ocean currents and upper layer water masses to a certain extent. This is the first study on relationship of foraminiferal eDNA and environmental factors in the Yellow Sea shelf region of the western Pacific Ocean, and it demonstrates that community parameters in foraminiferal eDNA could be a valuable proxy for environmental changes in the shelf sea.