Stratum corneum lipids, skin barrier function and filaggrin mutations in patients with atopic eczema
Stratum corneum lipids, skin barrier function and filaggrin mutations in patients with atopic eczema
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DOI：
10.1111/j.1398-9995.2010.02326.x
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发表时间：
2010-07-01
期刊：
影响因子：
12.4
通讯作者：
Weidinger, S.
中科院分区：
文献类型：
--
作者：
Jungersted, J. M.;Scheer, H.;Weidinger, S.
背景：在发现聚丝蛋白（FLG）突变之前，特应性皮炎（AD）患者皮肤屏障受损的证据已被记录，AD患者神经酰胺谱的变化，皮肤pH值的改变和经表皮水分丢失（TEWL）的增加也已被报道。到目前为止，没有研究分析角质层（SC）脂质结合已知的FLG genotype.Methods的主题与皮肤屏障参数：一个队列的49个德国人的基因型为最常见的FLG突变（R501 X，2282 del 4）的SC样品采取脂质分析的高效薄层色谱法。此外，测量TEWL、红斑、皮肤水合作用和pH。在49名个体中的27名中，使用十二烷基硫酸钠进行了24小时刺激斑贴试验。为了进行分析，AD组和对照组均按FLG突变状态（FLGmut/FLGwt）分层。结果：与两个健康对照组相比，在FLGmut AD组中，观察到神经酰胺4的水平显著降低，神经酰胺7的水平显著升高。然而，神经酰胺7水平在FLGwt AD和FLGwt对照之间也有显著差异，神经酰胺1水平也有显著差异。神经酰胺2、3、5和6未观察到显著差异。FLGmut个体的皮肤pH值显著高于未携带FLG突变的个体。与AD患者FLG突变有显着较高的红斑AD患者相比，没有FLG mutation.Conclusion：我们的研究结果证实了以前的观察改变神经酰胺水平在AD，但似乎没有显示出明确的关系与FLG突变。
P>Background:Prior to the discovery of filaggrin (FLG) mutations, evidence for an impaired skin barrier in atopic dermatitis (AD) has been documented, and changes in ceramide profile, altered skin pH and increased trans-epidermal water loss (TEWL) in patients with AD have been reported. Until now, no studies have analysed stratum corneum (SC) lipids combined with skin barrier parameters in subjects of known FLG genotype.Methods:A cohort of 49 German individuals genotyped for the most common FLG mutations (R501X, 2282del4) had SC samples taken for lipid analysis by high-performance thin layer chromatography. In addition, TEWL, erythema, skin hydration and pH were measured. In 27 of the 49 individuals, a 24-h irritation patch test with sodium lauryl sulphate was performed. For the analysis, both the AD group and the control group were stratified by FLG mutation status (FLGmut/FLGwt).Results:In the FLGmut AD group, significantly lower levels of ceramide 4 and significantly higher levels of ceramide 7 were observed when compared to both healthy control groups. However, ceramide 7 levels also significantly differed between FLGwt AD and FLGwt controls, as did ceramide 1 levels. No significant differences were observed for ceramide 2, 3, 5 and 6. FLGmut individuals had significantly higher skin pH values than individuals not carrying FLG mutations. Patients with AD with FLG mutations had significantly higher erythema compared to patients with AD without FLG mutations.Conclusion:Our results confirm previous observations of altered ceramide levels in AD, which however appear to show no clear relationship with FLG mutations.