IMMUNOREACTIVE FIBROBLAST GROWTH-FACTOR IN CELLS OF PERITONEAL-EXUDATE SUGGESTS ITS IDENTITY WITH MACROPHAGE-DERIVED GROWTH-FACTOR
IMMUNOREACTIVE FIBROBLAST GROWTH-FACTOR IN CELLS OF PERITONEAL-EXUDATE SUGGESTS ITS IDENTITY WITH MACROPHAGE-DERIVED GROWTH-FACTOR
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DOI：
10.1016/0006-291x(85)90614-x
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
BOHLEN, P
中科院分区：
文献类型：
--
作者：
BAIRD, A;MORMEDE, P;BOHLEN, P
从硫代乙酸酯刺激的小鼠的腹膜渗出液细胞中提取细胞，并检测其免疫活性和生物活性成纤维细胞生长因子(FGF)的存在。粗提物以剂量依赖的方式刺激牛主动脉弓来源的血管内皮细胞增殖。提取物还表现出平行的和剂量依赖的结合抑制作用，是一种对成纤维细胞生长因子具有高度特异性的放射免疫分析方法。提取液中含有的免疫活性成纤维细胞生长因子(ir-成纤维细胞生长因子)保留在肝素-琼脂糖亲和柱上，这是垂体型成纤维细胞生长因子的特点。巨噬细胞衍生材料的反相[高效液相色谱]显示1种生物活性形式的成纤维细胞生长因子，这与免疫反应的主要形式相一致。已证实在这些细胞中存在成纤维细胞生长因子；在这些提取物中至少有一种先前未知的有丝分裂活性显然是由于一种与成纤维细胞生长因子无法区分的有丝分裂原。
Peritoneal exudate cells were collected from thioglycollate stimulated mice, extracted and examined for the presence of immunoreactive and bioactivity fibroblast growth factor (FGF). The crude extract stimulated in a dose dependent fashion the proliferation of vascular endothelial cells derived from the bovine aortic arch. The extract also showed a parallel and dose-dependent inhibition of binding is a highly specific radioimmunoassay for FGF. The immunoreactive FGF (ir-FGF) contained in the extract was retained on a heparin-sepharose affinity column as is characteristic of pituitary FGF. Reverse-phase [high performance liquid chromatography] of the macrophage-derived material reveals 1 biologically active form of FGF which coelutes with the major form of immunoreactivity. The presence of FGF in these cells was demonstrated; at least 1 of the previously unidentified mitotic activities in these extracts apparently is due to a mitogen indistinguishable from FGF.