Biosensor based on acetylcholinesterase immobilized onto layered double hydroxides for flow injection/amperometric detection of organophosphate pesticides
Biosensor based on acetylcholinesterase immobilized onto layered double hydroxides for flow injection/amperometric detection of organophosphate pesticides
复制标题
基于固定在层状双氢氧化物上的乙酰胆碱酯酶的生物传感器，用于有机磷农药的流动注射/电流检测
DOI：
10.1016/j.bios.2012.07.026
复制
发表时间：
2013-01-15
影响因子：
12.6
通讯作者：
Song, Dandan
中科院分区：
文献类型：
--
作者：
Gong, Jingming;Guan, Zhangqiong;Song, Dandan
We developed a highly sensitive flow injection/amperometric biosensor for the detection of organophosphate pesticides (OPs) using layered double hydroxides (LDHs) as the immobilization matrix of acetylcholinesterase (AChE). LDHs provided a biocompatible microenvironment to keep the bioactivity of AChE, due to the intrinsic properties of LDHs (such as a regular structure, good mechanical, chemical and thermal stabilities, and swelling properties). By integrating the flow injection analysis (FIA) with amperometric detection, the resulting AChE-LDHs modified electrode greatly catalyzed the oxidation of the enzymatically generated thiocholine product, and facilitated the detection automation, thus increasing the detection sensitivity. The analytical conditions for the FIA/amperometric detection of OPs were optimized by using methyl parathion (MP) as a model. The inhibition of MP was proportional to its concentration ranging from 0.005 to 0.3 mu g mL(-1) and 0.3 to 4.0 mu g mL(-1) with a detection limit 0.6 ng mL(-1) (S/N=3). The developed biosensor exhibited good reproducibility and acceptable stability. (C) 2012 Elsevier B.V. All rights reserved.
