P450CAM - OXYGENATED COMPLEXES STABILIZED AT LOW-TEMPERATURE

P450CAM - OXYGENATED COMPLEXES STABILIZED AT LOW-TEMPERATURE
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DOI:
10.1016/s0006-291x(77)80131-9
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发表时间:
1977-01-01
影响因子:
3.1
通讯作者:
DOUZOU, P
DOUZOU, P
中科院分区:
生物学4区
文献类型:
--
作者:
EISENSTEIN, L;DEBEY, P;DOUZOU, P

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恶臭假单胞菌的亚铁细胞色素P450的氧化复合物,有或没有底物,进行自氧化。自发衰变率为一阶,降低了基板的存在下,并显着延迟在流体混合溶剂在零度以下的温度。如此稳定,两种络合物可用作纯的起始试剂。
Oxygenated complexes of the ferrous cytochrome P450 from Pseudomonas putida, with or without substrate, undergo autoxidation. The spontaneous decay rates are 1st order, decreased by the presence of substrate, and markedly retarded in fluid mixed solvents at sub-zero temperatures. Thus stabilized, both complexes may be used as pure starting reagents.