Two ATP Binding Cassette G Transporters, Rice ATP Binding Cassette G26 and ATP Binding Cassette G15, Collaboratively Regulate Rice Male Reproduction1[OPEN]
Two ATP Binding Cassette G Transporters, Rice ATP Binding Cassette G26 and ATP Binding Cassette G15, Collaboratively Regulate Rice Male Reproduction1[OPEN]
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DOI：
10.1104/pp.15.00262
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发表时间：
2015-09
期刊：
影响因子：
7.4
通讯作者：
Guochao Zhao;Jianxin Shi;W. Liang;Feiyang Xue;Qian Luo;Lu Zhu;Guorun Qu;Mingjiao Chen;L. Schre
中科院分区：
文献类型：
--
作者：
Guochao Zhao;Jianxin Shi;W. Liang;Feiyang Xue;Qian Luo;Lu Zhu;Guorun Qu;Mingjiao Chen;L. Schre
两个ATP结合盒G转运体在花药和花粉外壁发育过程中起协同作用。高等植物的雄性生殖需要各种代谢物的支持，包括花药壁最内层（绒毡层）中产生的脂质分子，但这些分子如何在不同的花药组织中分配仍然是未知的。以前，水稻（Oryza sativa）ATP结合盒G15（ABCG 15）及其拟南芥（Arabidopsis thaliana）的直系同源物被证明是花粉外壁形成所必需的。在此，我们报道了OsABCG 26与OsABCG 15一起在水稻中调节花药角质层和花粉外壁发育中的重要作用。细胞学和化学分析表明，osabcg26显示减少运输的分子从绒毡层细胞花药角质层发育。OsABCG26定位于花药壁细胞质膜上。相反，OsABCG 15极性定位于绒毡层质膜上，面向药室。osabcg26和osabcg15双突变体与osabcg15单突变体相似，几乎完全没有花药角质层和花粉外壁。综上所述，我们认为OsABCG26和OsABCG15协同调控水稻雄性生殖：OsABCG26主要负责将绒毡层细胞中的外源分子转运到花药壁层，而OsABCG15主要负责将外源分子从绒毡层细胞转运到花药室，以促进花粉外壁发育。
Two ATP binding cassette G transporters play a collaborative role in transferring lipidic molecules from tapetal cells for the development of anther cuticle and pollen exine. Male reproduction in higher plants requires the support of various metabolites, including lipid molecules produced in the innermost anther wall layer (the tapetum), but how the molecules are allocated among different anther tissues remains largely unknown. Previously, rice (Oryza sativa) ATP binding cassette G15 (ABCG15) and its Arabidopsis (Arabidopsis thaliana) ortholog were shown to be required for pollen exine formation. Here, we report the significant role of OsABCG26 in regulating the development of anther cuticle and pollen exine together with OsABCG15 in rice. Cytological and chemical analyses indicate that osabcg26 shows reduced transport of lipidic molecules from tapetal cells for anther cuticle development. Supportively, the localization of OsABCG26 is on the plasma membrane of the anther wall layers. By contrast, OsABCG15 is polarly localized in tapetal plasma membrane facing anther locules. osabcg26 osabcg15 double mutant displays an almost complete absence of anther cuticle and pollen exine, similar to that of osabcg15 single mutant. Taken together, we propose that OsABCG26 and OsABCG15 collaboratively regulate rice male reproduction: OsABCG26 is mainly responsible for the transport of lipidic molecules from tapetal cells to anther wall layers, whereas OsABCG15 mainly is responsible for the export of lipidic molecules from the tapetal cells to anther locules for pollen exine development.