DNA MODIFICATION OF A MAIZE TRANSPOSABLE ELEMENT CORRELATES WITH LOSS OF ACTIVITY
DNA MODIFICATION OF A MAIZE TRANSPOSABLE ELEMENT CORRELATES WITH LOSS OF ACTIVITY
复制标题
DOI：
10.1073/pnas.83.6.1767
复制
发表时间：
1986-03-01
影响因子：
11.1
通讯作者：
WALBOT, V
中科院分区：
文献类型：
--
作者：
CHANDLER, VL;WALBOT, V
An unstable allele of the bronze 2 (bz2) locus was isolated from a Robertson''s Mutator Zea mays line containing a family of active transposable Mu elements. This mutation is somatically unstable, resulting in numerous revertant purple sectors on a bronze kernel. By following the variegated kernel phenotype through two generation, several lineages have been identified that have a distorted transmission of the mutant phenotype (fewer variegated kernels are produced than expected). Southern blot analysis of Mu elements in these plants demonstrates a correlation between an inhibition of digestion of Mu elements by certain restriction enzymes and the loss of somatic reversion at the mutant allele. The DNA modification can occur in all the Mu elements in a plant within one generation; however, plants have been identified that contain both modified and unmodified elements, suggesting that the modification can occur in a progressive manner. We hypothesize that the DNA modification results in nonfunctional elements.
