Exposure to double-stranded RNA mediated by tobacco rattle virus leads to transcription up-regulation of effector gene Mi-vap-2 from Meloidogyne incognita and promotion of pathogenicity in progeny
Exposure to double-stranded RNA mediated by tobacco rattle virus leads to transcription up-regulation of effector gene Mi-vap-2 from Meloidogyne incognita and promotion of pathogenicity in progeny
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暴露于烟草脆裂病毒介导的双链 RNA 会导致南方根结线虫效应基因 Mi-vap-2 转录上调，并增强子代的致病性
DOI：
10.1016/j.ijpara.2015.09.006
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发表时间：
2016-02-01
影响因子：
4
通讯作者：
Li, Hongmei
中科院分区：
文献类型：
--
作者：
Chi, Yuankai;Wang, Xuan;Li, Hongmei
根结线虫属是经济上重要的植物寄生虫，对世界范围内的农业造成巨大损害。这些线虫使用分泌的效应物来修饰宿主细胞，使它们能够获得生长和发育所需的营养。深入了解效应子在线虫寄生中的作用对于了解线虫与寄主相互作用的机制具有重要意义。在这项研究中，通过烟草脆裂病毒介导的RNA干扰来靶向南方根结线虫的Mi-vap-2（编码毒液过敏原样蛋白的基因）。出乎意料的是，与野生型品系相比，在感染后7天收集的青少年中观察到Mi-vap-2转录物的显著上调。用TRV：：vap-2农杆菌渗入烟草。靶向转录物的这种上调不影响雌性的发育或虫瘿的产生，也不影响TRV：：vap-2系上的雌性数量。在阳性对照系中，在感染后7天，来自TRV：：16 D10系的幼体中M116 D10的转录物被敲低，导致线虫发育的显著抑制。由TRV-RNAi引发的Mi-vap-2的上调由暴露于双链RNA的线虫的后代遗传。同时，通过ELISA揭示了在这些幼年后代中Mi-VAP-2表达的显著增加。结果表明，在幼苗上产生的虫瘿和雌虫数量分别增加了71.2%和84.6%。与对照线虫产生的数量相比，本萨米亚那线虫产生的数量。因此，上调Mi-vap-2及其编码蛋白增强了线虫的致病性，表明Mi-vap-2可能是线虫在寄生早期成功寄生所必需的。无名氏(C)2015年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Meloidogyne spp. are economically important plant parasites and cause enormous damage to agriculture world-wide. These nematodes use secreted effectors which modify host cells, allowing them to obtain the nutrients required for growth and development. A better understanding of the roles of effectors in nematode parasitism is critical for understanding the mechanisms of nematode-host interactions. In this study, Mi-vap-2 of Meloidogyne incognita, a gene encoding a venom allergen-like protein, was targeted by RNA interference mediated by the tobacco rattle virus. Unexpectedly, compared with a wild type line, a substantial up-regulation of Mi-vap-2 transcript was observed in juveniles collected at 7 days p.i. from Nicotiana benthamiana agroinfiltrated with TRV::vap-2. This up-regulation of the targeted transcript did not impact development of females or the production of galls, nor the number of females on the TRV::vap-2 line. In a positive control line, the transcript of Mi16D10 was knocked down in juveniles from the TRV::16D10 line at 7 days p.i., resulting in a significant inhibition of nematode development. The up-regulation of Mi-vap-2 triggered by TRV-RNAi was inherited by the progeny of the nematodes exposed to double-stranded RNA. Meanwhile, a substantial increase in Mi-VAP-2 expression in those juvenile progeny was revealed by ELISA. This caused an increase in the number of galls (71.2%) and females (84.6%) produced on seedlings of N. benthamiana compared with the numbers produced by control nematodes. Up-regulation of Mi-vap-2 and its encoded protein therefore enhanced pathogenicity of the nematodes, suggesting that Mi-vap-2 may be required for successful parasitism during the early parasitic stage of M. incognita. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.