Use of Bone Morphogenetic Protein 2 on Ectopic Porous Coated Implants in the Rat
Use of Bone Morphogenetic Protein 2 on Ectopic Porous Coated Implants in the Rat
复制标题
骨形态发生蛋白 2 在大鼠异位多孔涂层植入物上的应用
DOI：
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发表时间：
1997
影响因子：
4.2
通讯作者：
A. Weiland
中科院分区：
文献类型：
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作者：
B. Cole;M. Bostrom;Traci L. Pritchard;D. Sumner;E. Tomin;J. Lane;A. Weiland
The ability of recombinant human bone morphogenetic protein 2 to remain osteoinductive and stimulate appositional bone formation on a porous coated implant was tested in a rat quadriceps muscle pouch. Implants with or without hydroxyapatite were used to compare the effects on bone formation of two different doses (23 μg or 46 μg) of recombinant human bone morphogenetic protein 2 against controls as evidenced by contact radiography, histologic examination, and backscatter scanning electron microscopic analysis. Cylindrical plasma sprayed porous titanium implants were placed bilaterally within a muscle pouch surgically created in 48 Lewis rats. Implants treated with recombinant human bone morphogenetic protein 2 formed significantly more bone than did control implants independent of the dose or presence of hydroxyapatite. In all implants with bone formation, osteoinduction via endochondral ossification began within 7 days. By 21 days, cartilage largely was replaced by bone and marrow. The results of this ectopic, nonweightbearing in vivo assay suggest that recombinant human bone morphogenetic protein 2 remains biologically active after application to a titanium implant and may be used to enhance appositional bone formation by direct application to the implant surface.
