Rice Defense mechanisms against Cochliobolus miyabeanus and Magnaporthe grisea are distinct
Rice Defense mechanisms against Cochliobolus miyabeanus and Magnaporthe grisea are distinct
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DOI：
10.1094/phyto-95-1248
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发表时间：
2005-11-01
期刊：
影响因子：
3.2
通讯作者：
Lee, YH
中科院分区：
文献类型：
--
作者：
Ahn, P;Kim, S;Lee, YH
比较了水稻对稻瘟病菌和棉铃虫的反应。在对这两种真菌都有抗性的水稻品种Tetep中，病原菌的接种迅速引发了过敏性反应(HR)，导致微观细胞死亡。在大米简历中。对这两种病原菌都敏感的稻瘟病菌不会引起HR，而Miybeanus引起的细胞迅速死亡与FIR相似，似乎与cv.Tetep，但未能阻断真菌的分支。小麦分生孢子萌发液(CGF)处理诱导两个品种的细胞迅速死亡，表明CGF预处理中存在植物毒素。Nakdon+CGF显著提高了对稻瘟病菌的抗性，而相同处理对稻瘟病菌的抗性无明显影响。同样，在简历中。Nakdon、苯并噻二唑(BTH)显著提高了对稻瘟病菌的抗性，但对稻瘟病菌的抗性不明显。茉莉酸甲酯(MeJA)处理似乎对这两种真菌都无效。增加了变种的阻力。BTH或CCF对稻瘟病菌的诱导与3个被监测的PR基因的快速诱导有关。MeJA的应用导致JAmyb在cv.对稻瘟病菌侵染的反应比未经处理的植株诱导得更快。相反，PR和JAmyb基因在不同CVS之间的表达模式几乎相同。Nakdon和Tetep，BTH和MeJA处理都没有显著改变它们的表达模式，以响应米氏弧菌的感染。我们的结果表明，水稻对稻瘟病菌和稻瘟病菌具有不同的防御机制。
Responses of rice to Magnaporthe grisea and Cochliobolus miyabeanus were compared. In Tetep, a rice cultivar resistant to both fungi, pathogen inoculation rapidly triggered the hypersensitive reaction (HR), resulting in microscopic cell death. In rice cv. Nakdong, susceptible to both pathogens, M. grisea did not cause HR, whereas C. miyabeanus caused rapid cell death similar to that associated with FIR, which appeared similar to that observed in cv. Tetep, yet failed to block fungal ramification. Treatment with conidial germination fluid (CGF) from C. miyabeanus induced rapid cell death in both cultivars, suggesting the presence of phytotoxins in CGF Pretreatment of cv. Nakdong with CGF significantly increased resistance to M. grisea, while the same treatment was ineffective against C. miyabeanus. Similarly, in cv. Nakdong, benzo-thiadiazole (BTH) significantly increased resistance to M. grisea, but was ineffective against C. miyabeanus. Methyl jasmonate (MeJA) treatment appeared to be ineffective against either fungus. Increased resistance of cv. Nakdong to M. grisea by BTH or CCF treatment was correlated with more rapid induction of three monitored PR genes. Application of MeJA resulted in the expression of JAmyb in cv. Nakdong being induced faster than in untreated plants in response to M. grisea infection. In contrast, the expression pattern of the PR and JAmyb genes in response to C. miyabeanus was nearly identical between cvs. Nakdong and Tetep, and neither BTH nor MeJA treatment significantly modified their expression patterns in response to C. miyabeanus infection. Our results suggest that rice employs distinct mechanisms for its defense against M. grisea versus C. miyabeanus.