QTL-seq identifies major quantitative trait loci of stigma color in melon
QTL-seq identifies major quantitative trait loci of stigma color in melon
复制标题
QTL-seq识别甜瓜柱头颜色的主要数量性状位点
DOI：
10.1016/j.hpj.2021.01.004
复制
发表时间：
2021-07-01
影响因子：
5.7
通讯作者：
Luan, Feishi
中科院分区：
文献类型：
--
作者：
Qiao, Aohan;Fang, Xufeng;Luan, Feishi
Stigma color plays an important role in pollination. In nature, melon (Cucumis melo L.) stigmas are either yellow or green; however, a review of the literature found no report on how stigma color affects pollination and fruit development in melon. Here, we used an F-2 melon population derived from a cross between 'MR-1' (P-1, with green stigmas) and 'M1-32' (P-2, with yellow stigmas), and performed genetic analysis and mapping. The results of bulked segregant analysis allowed the identification of genetic loci controlling stigma color on chromosomes 6 and 8. An F-2 population consisting of 150 individuals was used for initial mapping. A genetic map of 304.17 cM was constructed using 37 cleaved amplified polymorphism sequence (CAPS) markers. We identified one major quantitative trait locus (QTL) and one minor QTL for stigma color. The major QTL GS8.1 was further mapped to a 4.13 cM interval between CAPS markers 8C-10 and 8C-16, which explained 27.04% of the phenotypic variation. In addition, GS6.1 was mapped between E-49 and 6A-7, explaining 18.6% of the phenotypic variation. This study provides a theoretical basis for the fine mapping and cloning of melon genes controlling stigma color.
