In Vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates
In Vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates
复制标题
无菌颗粒反应中性粒细胞募集的体内评估
DOI：
10.1007/978-1-62703-523-1_16
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hajime Kono
中科院分区：
文献类型：
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作者：
Tamiko Yanagida;Kaoru Orihashi;Hajime Kono
尿酸钠晶体等无菌颗粒会诱导炎症小体激活，从而导致 caspase-1 激活、IL-1α 分泌和 IL-1β 加工。 IL-1 的局部产生和激活导致中性粒细胞在体内募集。在这里，我们描述了两种快速而简单的方法，用于评估腹膜腔和皮肤中中性粒细胞对无菌颗粒的反应，这依赖于 IL-1 受体信号传导。
Sterile particulates such as monosodium urate crystals induce inflammasome activation resulting in activation of caspase-1, secretion of IL-1α, and processing of IL-1β. Local production and activation of IL-1 leads to neutrophil recruitment in vivo. Here we describe two quick and simple methods for the evaluation of neutrophil recruitment in the peritoneal cavity and skin in response to sterile particulates, which are dependent on IL-1 receptor signaling.