SSR Marker of Wheat Stripe Rust Resistance Gene Yr2
SSR Marker of Wheat Stripe Rust Resistance Gene Yr2
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发表时间：
2005
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通讯作者：
L. Lan
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作者：
L. Lan
本研究旨在为Yr 2基因的有效利用和基于紧密连锁的Yr 2基因克隆提供参考。利用近等基因系台中29 * 6/海恩斯-麦与感病亲本台中29建立F2分离群体，并进行苗期抗条锈性鉴定。遗传分析表明，台中29 * 6/海恩斯小麦的抗条锈性受单基因控制。利用位于7 B染色体上的45对SSR引物，对F2分离群体及其抗、感病亲本的171个单株进行SSR分析，筛选出与抗条锈基因Yr 2连锁的分子标记WMC 364 - 207 bp/201 bp。标记WMC 364 - 207 bp/201 bp与抗病基因Yr 2之间的遗传距离为5。6厘米。
This study was to facilitate the effective use of Yr2 gene and Yr2 gene cloning based on tight linkage. Using near isogenic line Taichung 29 * 6/Heines Ⅶ and Susceptible parent Taichung 29, we established F2 segregation population and identified its stripe rust resistance in seeding stage. Genetic analysis suggested that the stripe rust resistance Taichung 29 * 6/Heines Ⅶ was controlled by a single gene. By SSR analysis with 45 pairs of primers located on 7B chromesome in 171 individuals of F2 segregation population and their resistant and susceptible parents, we screened a molecular marker WMC364-207bp/201bp that linked to the stripe rust resistance gene Yr2. Genetic distance among marker WMC364-207bp/201bp and resistance gene Yr2 was calculated as 5. 6 cM.