Quantitative PCR assays for mouse enteric flora reveal strain-dependent differences in composition that are influenced by the microenvironment
Quantitative PCR assays for mouse enteric flora reveal strain-dependent differences in composition that are influenced by the microenvironment
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DOI：
10.1007/s00335-006-0063-1
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发表时间：
2006-11-01
期刊：
影响因子：
2.5
通讯作者：
Threadgill, David W.
中科院分区：
文献类型：
--
作者：
Alexander, A. Deloris;Orcutt, Roger P.;Threadgill, David W.
哺乳动物的胃肠道中有超过一百种的共生菌。个体间GI植物群组成的差异可能导致体内实验分析和疾病易感性的变化。为了研究GI植物群组成的潜在个体间差异，我们开发了基于实时定量PCR的检测方法，用于检测作为哺乳动物胃肠道内不同细菌生态位代表性成员的8种改变的Schaedler植物群（ASF）。在23种不同屏障饲养的近交系小鼠品系中观察到ASF成员数量的定量和可重复的品系特异性变化，表明ASF测定可用作GI植物群组成变化的哨兵。还检测到显著的笼效应。在断奶时同居的同基因小鼠，无论是来自相同或不同的窝，在ASF配置文件中显示出很小的变化。相反，断奶时在不同笼中分割的窝仔在三周后显示ASF特征的差异。个体ASF谱一旦建立，在没有环境扰动的情况下随着时间的推移高度稳定。此外，不同的近交系的同居保持了大部分的菌株间的GI植物群的变化，支持宿主遗传学的作用，在确定GI植物群组成。
The mammalian gastrointestinal (GI) tract is inhabited by over a hundred species of symbiotic bacteria. Differences among individuals in the composition of the GI flora may contribute to variation in in vivo experimental analyses and disease susceptibility. To investigate potential interindividual differences in GI flora composition, we developed real-time quantitative PCR-based assays for the detection of the eight members of the Altered Schaedler Flora (ASF) as representative members of different bacterial niches within the mammalian GI tract. Quantitative and reproducible strain-specific variations in the numbers of the ASF members were observed across 23 different barrier-housed inbred mouse strains, suggesting that the ASF assays can be used as sentinels for changes in GI flora composition. A significant cage effect was also detected. Isogenic mice that cohabited at weaning, whether from the same or different litters, showed little variation in ASF profiles. Conversely, litters split among different cages at weaning showed divergence in ASF profiles after three weeks. Individual ASF profiles, once established, were highly stable over time in the absence of environmental perturbation. Furthermore, cohabitation of different inbred strains maintained most of the interstrain variation in the GI flora, supporting a role of host genetics in determining GI flora composition.