Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)
Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)
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DOI：
10.1073/pnas.0906970106
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发表时间：
2009-09-01
影响因子：
11.1
通讯作者：
Berenbaum, May R.
中科院分区：
文献类型：
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作者：
Johnson, Reed M.;Evans, Jay D.;Berenbaum, May R.
蜂群衰竭失调症（Colony Collapse Disorder，CCD）是一种蜜蜂的神秘消失，自2006年底以来一直困扰着美国的养蜂人。病原体和包括杀虫剂在内的其他环境压力与CCD有关，但因果关系尚未得到证实。由于肠道作为蜜蜂与其环境之间的主要界面，作为病原体和毒素的进入场所，我们使用全基因组微阵列来比较源自美国东海岸和西海岸的CCD殖民地的蜜蜂肠道与CCD出现之前采样的健康殖民地的蜜蜂肠道之间的基因表达。相当大的差异与蜜蜂的地理来源的基因表达，但65个成绩单的共识列表被确定为CCD状态的潜在标记。总体而言，没有观察到农药反应基因的表达升高。参与免疫应答的基因在表达模式中没有显示出明显的趋势，尽管CCD菌落中病毒和其他病原体的流行率增加。微阵列分析显示，不寻常的核糖体RNA片段，显着更丰富的CCD蜜蜂的肠道。这些片段的存在可能是小核糖核酸样病毒感染的结果，包括变形翅病毒和以色列急性麻痹病毒，并可能与停滞翻译有关。核糖体片段的丰度和存在的多种病毒可能被证明是有用的诊断标记与CCD困扰的殖民地。
Colony collapse disorder (CCD) is a mysterious disappearance of honey bees that has beset beekeepers in the United States since late 2006. Pathogens and other environmental stresses, including pesticides, have been linked to CCD, but a causal relationship has not yet been demonstrated. Because the gut acts as a primary interface between the honey bee and its environment as a site of entry for pathogens and toxins, we used whole-genome microarrays to compare gene expression between guts of bees from CCD colonies originating on both the east and west coasts of the United States and guts of bees from healthy colonies sampled before the emergence of CCD. Considerable variation in gene expression was associated with the geographical origin of bees, but a consensus list of 65 transcripts was identified as potential markers for CCD status. Overall, elevated expression of pesticide response genes was not observed. Genes involved in immune response showed no clear trend in expression pattern despite the increased prevalence of viruses and other pathogens in CCD colonies. Microarray analysis revealed unusual ribosomal RNA fragments that were conspicuously more abundant in the guts of CCD bees. The presence of these fragments may be a possible consequence of picorna-like viral infection, including deformed wing virus and Israeli acute paralysis virus, and may be related to arrested translation. Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD.