SIC: an intracerebral radiosensitive probe for in vivo neuropharmacology investigations in small laboratory animals: first prototype design, characterization and in vivo evaluation
SIC: an intracerebral radiosensitive probe for in vivo neuropharmacology investigations in small laboratory animals: first prototype design, characterization and in vivo evaluation
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SIC：用于小型实验动物体内神经药理学研究的脑内放射敏感探针：第一个原型设计、表征和体内评估
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
L. Valentin
中科院分区：
文献类型：
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作者：
F. Pain;P. Laniece;R. Mastrippolito;L. Pinot;Y. Charon;A. Glatigny;M. T. Guillemin;Philippe Hantraye;Vincent Leviel;L. Ménard;L. Valentin
目前正在开发几种专用于小动物成像的高分辨率断层摄影机。然而，这些装置有两个主要缺点：它们的成本高，需要动物麻醉和固定，从而限制了研究领域。因此，作者提出了SIC（法语“Sonde IntraCerebrale”的首字母缩写），这是一种低成本的β微探针，能够在清醒、自由活动的动物体内局部计数放射性浓度。除了良好的灵敏度，该工具提供了一个高的时间分辨率的优点，在体内测量示踪剂动力学的一个主要资产。此外，它可以很容易地与现有的技术，如电生理学，微透析，伏安法和磁共振成像，从而同时提供补充信息的大脑机制。作者在这里介绍了第一个原型的设计，制造和第一次评估。第一次体外评价使用一个简单的烧杯充满了均匀的水溶液[/sup 18/F]-氟脱氧葡萄糖（FDG）证实了作者的第一个模拟结果的灵敏度和检测体积。然后，进一步的实验进行了体内麻醉大鼠轴承两个植入探针在大脑中验证了使用SIC测量本地，具有高的时间分辨率，放射性标记的分子在活的啮齿动物的浓度。
Several high-resolution tomographs dedicated to small animal imaging are presently under development. However, these devices suffer from two major drawbacks: their cost is high and require animal anaesthesia and immobilization thus restricting the field of investigations. Therefore, the authors have proposed SIC (French acronym for "Sonde IntraCerebrale") a low cost beta microprobe able to count the radioactivity concentration locally in awake, freely moving animals. Beside a good sensitivity, this tool offers the advantage of a high temporal resolution, a major asset for in vivo measurements of tracer kinetics. Furthermore, it could be easily coupled with existing techniques such as electrophysiology, microdialysis, voltametry and magnetic resonance imaging thus providing simultaneously complementary information on the cerebral mechanisms. The authors present here the design, manufacture and first evaluations of a first prototype. A first in vitro evaluation using a simple beaker filled with an homogenous aqueous solution of [/sup 18/F]-fluorodeoxyglucose (FDG) confirmed the authors' first simulation results in terms of sensitivity and detection volume. Then, further experimentation carried out in vivo on anaesthetized rats bearing two implanted probes in the brain have validated the use of SIC to measure locally, with a high temporal resolution, the concentration of radiolabeled molecules in living rodents.
DOI：
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发表时间：
1999-07
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
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作者：
A. Chatziioannou;S. Cherry;Y. Shao;R. Silverman;K. Meadors;T. Farquhar;Marjan Pedarsani;M. Phelps
通讯作者：
A. Chatziioannou;S. Cherry;Y. Shao;R. Silverman;K. Meadors;T. Farquhar;Marjan Pedarsani;M. Phelps