A TISSUE-CULTURE INDUCED ADH1 NULL MUTANT OF MAIZE RESULTS FROM A SINGLE BASE CHANGE
A TISSUE-CULTURE INDUCED ADH1 NULL MUTANT OF MAIZE RESULTS FROM A SINGLE BASE CHANGE
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DOI：
10.1007/bf00337777
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发表时间：
1987-11-01
期刊：
影响因子：
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通讯作者：
PEACOCK, WJ
中科院分区：
文献类型：
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作者：
DENNIS, ES;BRETTELL, RIS;PEACOCK, WJ
在携带Adh1- 1s和Adh1- 1f等位基因的玉米胚组织培养后，我们发现了一个Adh1空等位基因作为体细胞无性系变异出现。克隆和测序结果表明，突变等位基因来源于Adh1-1S，该基因编码区出现了一个碱基变化，将AAG赖氨酸密码子转化为TAG终止密码子。核苷酸替换率（每218个培养的胚胎中有两个）比正常突变率要高得多。
We have found a null Adh1 allele which arose as a somaclonal variant following tissue culture of maize embryos carrying Adh1-1S and Adh1-1F alleles. Cloning and sequencing shows that the mutant allele derives from Adh1-1S and that there has been a single base change in the coding region of the gene which converts an AAG lysine codon to a TAG stop codon. The rate of nucleotide substitution (two per 218 embryos cultured) is much greater than normal mutation rates.