Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress
Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress
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DOI：
10.1016/s0008-6363(00)00174-7
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发表时间：
2000-10-01
影响因子：
10.8
通讯作者：
Jern, S
中科院分区：
文献类型：
--
作者：
Gan, LM;Selin-Sjögren, L;Jern, S
目的：通过使用计算机血管灌注模型，我们研究了亚急性压力升高对完整人体导管中血管舒缩行为以及内皮素-1 (ET-1) 和内皮一氧化氮合酶 (eNOS) 表达的时间影响。方法：在高/低腔内压力（40/20 mmHg）和相同的10 dyn/cm(2)剪切应力水平下，对成对脐静脉进行1.5、3和6小时的灌注。分别通过实时逆转录聚合酶链反应和定量免疫组织化学对 ET-1 和 eNOS 基因和蛋白表达进行定量。结果：压力诱导 ET-1 和 eNOS 基因表达的差异时间调节模式。在高压条件下，eNOS mRNA在灌注3小时后上调，并在灌注6小时后趋于平稳，而ET-1 mRNA在灌注6小时后升高。免疫组织化学验证了蛋白质水平的同步变化。在高压系统中3小时后观察到显着的血管舒张。结论：因此，亚急性压力升高对内皮细胞 eNOS/ET-1 表达产生不同的影响，从而动态调节血管舒缩张力。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Objective: By using a computerized vascular perfusion model, we investigated temporal effects of sub-acute pressure elevation on vasomotor behavior and expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) in intact human conduit vessels. Methods: Paired umbilical veins were perfused during 1.5, 3 and 6 h under high/low intraluminal pressure (40/20 mmHg) and at identical shear stress level of 10 dyn/cm(2). ET-1 and eNOS gene and protein expression was quantified with real-time reverse-transcribed polymerase chain reaction and quantitative immunohistochemistry, respectively. Results: Pressure induced differential temporal regulation patterns of ET-1 and eNOS gene expression. During the high pressure condition, eNOS mRNA was upregulated after 3 h and leveled off after 6 h of perfusion, while ET-1 mRNA was elevated after 6 h perfusion. Immunohistochemistry verified synchronal changes at the protein level. Significant vasodilation was observed after 3 h in the high-pressure system. Conclusion: Thus, subacute pressure elevation exerts differential effects on the endothelial eNOS/ET-1 expression, which dynamically regulate the vasomotor tone. (C) 2000 Elsevier Science B.V. All rights reserved.