Next is now: new technologies for sequencing of genomes, transcriptomes, and beyond.

Next is now: new technologies for sequencing of genomes, transcriptomes, and beyond.
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DOI:
10.1016/j.pbi.2008.11.004
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发表时间:
2009-04
影响因子:
9.5
通讯作者:
Ecker, Joseph R.
Ecker, Joseph R.
中科院分区:
生物学2区
文献类型:
--
作者:
Lister, Ryan;Gregory, Brian D.;Ecker, Joseph R.

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快速产生大量序列信息的DNA测序技术的突然可用性引发了基因组学的范式转变,使复杂核酸群体的大规模并行调查成为可能。这些技术已经应用到的应用的多样性证明了细胞过程和性质的巨大范围,现在可以在单碱基分辨率下进行研究。这些包括基因组重测序和多态性发现、突变作图、DNA甲基化、组蛋白修饰、转录组测序、基因发现、可变剪接鉴定、小RNA分析、DNA-蛋白质以及可能的蛋白质-蛋白质相互作用。因此,这些深度测序技术为植物生物学家提供了前所未有的机会,以增加对植物细胞和种群功能和动态的理解。
The sudden availability of DNA sequencing technologies that rapidly produce vast amounts of sequence information has triggered a paradigm shift in genomics, enabling massively parallel surveying of complex nucleic acid populations. The diversity of applications to which these technologies have already been applied demonstrates the immense range of cellular processes and properties that can now be studied at the single-base resolution. These include genome resequencing and polymorphism discovery, mutation mapping, DNA methylation, histone modifications, transcriptome sequencing, gene discovery, alternative splicing identification, small RNA profiling, DNA-protein and possibly even protein-protein interactions. Thus, these deep sequencing technologies offer plant biologists unprecedented opportunities to increase the understanding of the functions and dynamics of plant cells and populations.
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